Berberine inhibits norepinephrine-induced apoptosis in neonatal rat cardiomyocytes via inhibiting ROS-TNF-α-caspase signaling pathway
| Autor: | Chao-feng Hu, Yu-xia Yan, Yan-ping Wang, Xiuxiu Lv, Hua-dong Wang, Da-xiang Lu, Hongmei Li, Ren-bin Qi, Xiaohui Yu |
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| Rok vydání: | 2012 |
| Předmět: |
Berberine
Apoptosis Pharmacology Flow cytometry Rats Sprague-Dawley Norepinephrine (medication) Norepinephrine chemistry.chemical_compound medicine Animals Myocyte Myocytes Cardiac Pharmacology (medical) Annexin A5 Cell Shape Caspase Cell Nucleus L-Lactate Dehydrogenase medicine.diagnostic_test biology Caspase 3 Tumor Necrosis Factor-alpha Chemistry Caspase 2 DNA General Medicine Flow Cytometry Immunohistochemistry Rats Enzyme Activation Animals Newborn Complementary and alternative medicine Caspases biology.protein Signal transduction Reactive Oxygen Species Fluorescein-5-isothiocyanate Signal Transduction medicine.drug |
| Zdroj: | Chinese Journal of Integrative Medicine. 19:424-431 |
| ISSN: | 1993-0402 1672-0415 |
| DOI: | 10.1007/s11655-011-0856-2 |
| Popis: | To determine the effect of berberine (Ber) on norepinephrine (NE)-induced apoptosis in neonatal rat cardiomyocytes.The cultured neonatal rat cardiomyocytes were treated with NE in the presence or absence of Ber. The activity of lactate dehydrogenase (LDH) in the culture medium was examined, and apoptosis of cardiomyocytes was assessed by Hoechst 33258, isothiocyanate (FITC)-conjugated annexin-V, and propidine iodide (PI) staining. In addition, the activities of caspases-2 and-3 were measured by a fluorescent assay kit. The level of secreted tumor necrosis factor α (TNF-α) and production of intracellular reactive oxygen species (ROS) were also determined.NE at a concentration of 50 μ mol/L induced an obvious increase in the activity of LDH in the culture medium (P0.05), which was inhibited by coincubation with 0.5, 1.0, or 2.0 μ mol/L Ber (P0.05). Ber also significantly attenuated NE-induced apoptosis in a dose-dependent manner (P0.01). Moreover, Ber at a dose of 2 μ mol/L markedly decreased the ROS and TNF-α productions (P0.05) and inhibited the activation of caspases-2 and -3 in cardiomyocytes exposed to NE (P0.05)h.The present study suggested that Ber could reduce NE-induced apoptosis in neonatal rat cardiomyocytes through inhibiting the ROS-TNF-α-caspase signaling pathway. |
| Databáze: | OpenAIRE |
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