Single-cell amplified genomes of two uncultivated members of the deltaproteobacterial SEEP-SRB1 clade, isolated from marine sediment
| Autor: | Andreas Schramm, Kasper Urup Kjeldsen, Ian P. G. Marshall, Lars Schreiber, Caitlin Petro, Lara M. Jochum |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
SEEP-SRB Single-cell genomics SAGSEEP-SRB DIVERSITY Hydrocarbon degradation Aquatic Science 010603 evolutionary biology 01 natural sciences Genome REDUCTASE 03 medical and health sciences Genetics ARCHAEA TOOL PHYLOGENETIC ANALYSIS Clade Gene 030304 developmental biology 0303 health sciences biology Phylogenetic tree single-cell genomics Sediment 16S ribosomal RNA biology.organism_classification Petroleum seep Evolutionary biology SAG SULFATE-REDUCING BACTERIA sulfate-reducing bacteria ANAEROBIC OXIDATION Archaea hydrocarbon degradation |
| Zdroj: | Petro, C, Jochum, L M, Schreiber, L, Marshall, I P G, Schramm, A & Kjeldsen, K U 2019, ' Single-cell amplified genomes of two uncultivated members of the deltaproteobacterial SEEP-SRB1 clade, isolated from marine sediment ', Marine Genomics, vol. 46, pp. 66-69 . https://doi.org/10.1016/j.margen.2019.01.004 |
| DOI: | 10.1016/j.margen.2019.01.004 |
| Popis: | The SEEP-SRB1 is a diverse clade of sulfate reducing bacteria affiliated with the deltaproteobacterial family Desulfobacteraceae. Here we report the draft genome sequences of two single cells belonging to the SEEP-SRB1 clade, isolated from marine sediment in Aarhus Bay, Denmark, by fluorescence activated cell sorting (FACS). The draft genomes measure 2.12 Mb and 0.46 Mb, with GC contents of 45% and 42%. Phylogenetic placement of the 16S rRNA genes places the SAGs within the subgroups SEEP-SRB1e and SEEP-SRB1d. While these subgroups are thought to play a role in non-methane hydrocarbon degradation, there is little genomic information available to confirm their metabolic function. As such, these novel single-cell amplified genomes provide valuable insight into the metabolic potential of this prevalent subseafloor clade. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|