| Autor: |
Özkurt, Ezgi, Fritscher, Joachim, Soranzo, Nicola, Ng, Duncan Y. K., Davey, Robert P., Bahram, Mohammad, Hildebrand, Falk |
| Rok vydání: |
2022 |
| DOI: |
10.6084/m9.figshare.21359936 |
| Popis: |
Additional file 7: Supplementary Figure S3. Reproducibility of the technical replicates respective to different LotuS2 non-default parameters. Bray-Curtis distances between technical replicates of A) gut-16S, B) soil-16S, and C) soil-ITS datasets using default and non-default parameters (LotuS2 flags: -lulu 0, -xtalk 1, -keepUnclassified 1, -ITSx 0, where 1 means the option is activated; 0 means deactivated). When activated, -lulu option uses LULU R package [24] to merge OTUs/ASVs based on their co-occurrences; -xtalk option checks for cross-talk [33], -keepUnclassified includes unclassified (i.e. not matching to any taxon in the taxonomy database) OTUs/ASVs in the final matrix and –ITSx activates the ITSx program [32] to only retain OTUs fitting to ITS1/ITS2 hmm models. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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