Rat prolactin in serum, milk, and mammary tissue: characterization and intracellular localization

Autor: M Lkhider, S Delpal, M O Bousquet
Přispěvatelé: Laboratoire de nutrition et sécurité alimentaire, Institut National de la Recherche Agronomique (INRA), Unité de biologie cellulaire et moléculaire, ProdInra, Migration
Rok vydání: 1996
Předmět:
medicine.medical_specialty
Endosome
Immunoblotting
Fluorescent Antibody Technique
Biology
Immunofluorescence
Endocytosis
LOCALISATION
03 medical and health sciences
symbols.namesake
Mammary Glands
Animal
0302 clinical medicine
Endocrinology
Reference Values
Internal medicine
Lactation
medicine
Animals
Rats
Wistar
Microscopy
Immunoelectron
Bromocriptine
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
[SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism
0303 health sciences
medicine.diagnostic_test
Endoplasmic reticulum
Immunogold labelling
[SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism
Golgi apparatus
Prolactin
Rats
3. Good health
Molecular Weight
Milk
medicine.anatomical_structure
030220 oncology & carcinogenesis
symbols
RAT
Female
Subcellular Fractions
Zdroj: Endocrinology
Endocrinology, Endocrine Society, 1996, 137 (11), pp.4969-4979
ISSN: 1945-7170
0013-7227
DOI: 10.1210/endo.137.11.8895370
Popis: To study the localization of PRL in mammary epithelial cells (MEC) and to characterize PRL forms in serum, milk, and mammary tissue, two groups of lactating rats, a control group and a bromocriptine-treated group, were compared. In serum and milk from control rats, two forms of PRL (25 and 23 kDa) were detected by immunoblotting. In bromocriptine-treated rats, only the 25-kDa form was present. In mammary tissues from control rats, 25-, 23-, and 14-kDa forms of the hormone were detectable, whereas only 25- and 14-kDa forms were present in bromocriptine-treated rats. Immunofluorescence and immunogold electron microscopy revealed that in MEC from control rats, PRL was located in the organelles involved in endocytosis and was also present in rough endoplasmic reticulum, Golgi apparatus, secretory vesicles, and lumen of the acini. In bromocriptine-treated rats, a decrease in the labeling was evidenced in endosomes and multivesicular bodies. On the contrary, labeling associated with the rough endoplasmic reticulum and secretory vesicles was increased. Thus, even when the amount of circulating PRL detected by RIA was strongly decreased, PRL was always detectable in MEC and in the lumen of the acini, suggesting an active participation of MEC in the transport of PRL to milk.
Databáze: OpenAIRE
Externí odkaz: