OPS vitrification of mouse immature oocytes before or after meiosis: the effect on cumulus cells maintenance and subsequent development
| Autor: | Xue-Ming Zhao, Qing-Jing Zhang, Shi-En Zhu, Lun Suo, Qinggang Meng, Xiangwei Fu, Zhiqiang Fan, Yan-Ping Wang, Guang-Bin Zhou, Chang-Liang Yan |
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| Rok vydání: | 2009 |
| Předmět: |
endocrine system
medicine.medical_treatment Fertilization in Vitro Cryopreservation Andrology Mice Cryoprotective Agents Meiosis medicine Animals Vitrification Blastocyst Cumulus Cells Germinal vesicle In vitro fertilisation urogenital system Chemistry Cell Biology Oocyte In vitro medicine.anatomical_structure Oocytes Female Developmental Biology |
| Zdroj: | Zygote. 17:71-77 |
| ISSN: | 1469-8730 0967-1994 |
| DOI: | 10.1017/s0967199408005091 |
| Popis: | SummaryCryopreservation can cause cumulus cell damage around the immature oocytes, which may result in poor subsequent development. To evaluate the effect of the meiosis stage on the cumulus cell cryoinjury and determine the suitable stage for cryopreservation in immature oocytes, mouse oocytes at germinal vesicle (GV) and germinal vesicle breakdown (GVBD) stages were vitrified using open pulled straw (OPS) method. Cumulus cells damage was scored immediately after thawing by double-fluorescent staining. The survival rate of the oocytes was evaluated and the subsequent development of oocytes was assessed through in vitro culture (IVC) and in vitro fertilization (IVF) separately. After vitrification, a higher proportion of cumulus cells of GV oocytes were damaged than those of GVBD and untreated control groups. The survival rate of vitrified GVBD oocytes (94.1%) was significantly higher (p < 0.05) than that of GV oocytes (85.4%). Oocytes vitrified at GVBD stage (55.7%) showed similar cleavage rate compared to those at GV stage (49.2%), but significantly higher (p < 0.05) blastocyst rate (40.9% vs. 27.4%). These results demonstrate that oocytes at GVBD stage remain better cumulus membrane integrity and developmental ability during vitrification than those at GV stage, indicating they are more suitable for immature oocytes cryopreservation in mice. |
| Databáze: | OpenAIRE |
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