Global correlation of genome and transcriptome changes in classical Hodgkin lymphoma
| Autor: | Pieter van der Vlies, Anke van den Berg, Tjasso Blokzijl, Cigdem Atayar, Debora de Jong, Klaas Kok, Ralf Kueppers, Ines Pfeil, Geert Harms, Arjan Diepstra, Joost Kluiver, Sibrand Poppema |
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| Přispěvatelé: | Stem Cell Aging Leukemia and Lymphoma (SALL), Translational Immunology Groningen (TRIGR), Guided Treatment in Optimal Selected Cancer Patients (GUTS) |
| Rok vydání: | 2007 |
| Předmět: |
HLA CLASS-I
Cancer Research array-based CGH NF-KAPPA-B Down-Regulation CENTER B-CELLS Biology IRAK1 SAGE Genome DISEASE Transcriptome HLA Antigens Cell Line Tumor Gene expression ANTIGEN PRESENTATION medicine Humans RNA Neoplasm Serial analysis of gene expression REED-STERNBERG CELLS Gene B cell GENE-EXPRESSION Genetics CLONAL IMMUNOGLOBULIN MUTATIONS Gene Expression Profiling Microfilament Proteins Nucleic Acid Hybridization Hematology General Medicine Janus Kinase 2 medicine.disease Hodgkin Disease Molecular biology Proto-Oncogene Proteins c-rel Up-Regulation Interleukin-1 Receptor-Associated Kinases medicine.anatomical_structure Oncology Reed–Sternberg cell Carrier Proteins FASCIN Hodgkin lymphoma Genes Neoplasm FSCN1 Comparative genomic hybridization |
| Zdroj: | HEMATOLOGICAL ONCOLOGY, 25(1), 21-29. Wiley |
| ISSN: | 1099-1069 0278-0232 |
| DOI: | 10.1002/hon.804 |
| Popis: | To identify genes involved in the pathogenesis of classical Hodgkin lymphoma (cHL), we performed serial analysis of gene expression (SAGE) and array-based comparative genomic hybridization (aCGH). Comparison of SAGE libraries of cHL cell lines L428 and L1236 with that of germinal centre B cells revealed consistent overexpression of only 14 genes. In contrast, 141 genes were downregulated in both cHL cell lines, including many B cell and HLA genes. aCGH revealed gain of 2p, 7p, 9p, 11q and Xq and loss of 4q and 11q. Eighteen percent of the differentially expressed genes mapped to regions with loss or gain and a good correlation was observed between underexpression and loss or overexpression and gain of DNA. Remarkably, gain of 2p and 9p did not correlate with increased expression of the proposed target genes c-REL and JAK2. Downregullation of many genes within the HLA region also did not correlate with loss of DNA. FSCN1 and IRAK1 mapping at genomic loci (7p and Xq) that frequently showed gain were overexpressed in cHL cell lines and might be involved in the pathogenesis of cHL. Copyright (c) 2006 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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