A decrease of the butyrate-producing speciesRoseburia hominisandFaecalibacterium prausnitziidefines dysbiosis in patients with ulcerative colitis
| Autor: | Marc Ferrante, Venessa Eeckhaut, Vera Ballet, Marie Joossens, Paul Rutgeerts, João Sabino, Severine Vermeire, Jan Verhaegen, Filip Van Immerseel, Ingrid Arijs, Kathleen Machiels, Vicky De Preter, Karolien Claes, Kristin Verbeke |
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| Přispěvatelé: | Department of Bio-engineering Sciences, Faculty of Arts and Philosophy |
| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Firmicutes Faecalibacterium prausnitzii Gut flora Real-Time Polymerase Chain Reaction Roseburia hominis Severity of Illness Index Inflammatory bowel disease Microbiology Feces Gram-Negative Anaerobic Straight Curved and Helical Rods medicine Humans Lactic Acid Crohn's disease biology Denaturing Gradient Gel Electrophoresis Gastroenterology Middle Aged biology.organism_classification medicine.disease Ulcerative colitis Bacterial Load Case-Control Studies Immunology Butyric Acid Dysbiosis Colitis Ulcerative Female Propionates |
| Zdroj: | Gut. 63:1275-1283 |
| ISSN: | 1468-3288 0017-5749 |
| DOI: | 10.1136/gutjnl-2013-304833 |
| Popis: | OBJECTIVE: Bacteria play an important role in the onset and perpetuation of intestinal inflammation in inflammatory bowel disease (IBD). Unlike in Crohn's disease (CD), in which dysbiosis has been better characterised, in ulcerative colitis (UC), only small cohorts have been studied and showed conflicting data. Therefore, we evaluated in a large cohort if the microbial signature described in CD is also present in UC, and if we could characterise predominant dysbiosis in UC. To assess the functional impact of dysbiosis, we quantified the bacterial metabolites. DESIGN: The predominant microbiota from 127 UC patients and 87 age and sex-matched controls was analysed using denaturing gradient gel electrophoresis (DGGE) analysis. Differences were quantitatively validated using real-time PCR. Metabolites were quantified using gas chromatography-mass spectrometry. RESULTS: Based on DGGE analysis, the microbial signature previously described in CD was not present in UC. Real-time PCR analysis revealed a lower abundance of Roseburia hominis (p |
| Databáze: | OpenAIRE |
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