Ruminal microbe of biohydrogenation of trans-vaccenic acid to stearic acid in vitro
Autor: | Jia Qi Wang, Dan Li, Deng Pan Bu |
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Rok vydání: | 2012 |
Předmět: |
Chromatography
Gas Biohydrogenation Microbial Consortia Microbial metabolism lcsh:Medicine Oleic Acids Biology Ruminal microbe Enrichment culture General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Trans-vaccenic acid Biotransformation RNA Ribosomal 16S Animals lcsh:Science (General) lcsh:QH301-705.5 Phylogeny Unsaturated fatty acid Medicine(all) chemistry.chemical_classification Bacteria Biochemistry Genetics and Molecular Biology(all) Denaturing Gradient Gel Electrophoresis Stomach Ruminant lcsh:R food and beverages Fatty acid Ruminants General Medicine biology.organism_classification Culture Media lcsh:Biology (General) chemistry Biochemistry Hydrogenation Stearic acid Stearic Acids Temperature gradient gel electrophoresis Research Article lcsh:Q1-390 |
Zdroj: | BMC Research Notes, Vol 5, Iss 1, p 97 (2012) BMC Research Notes |
ISSN: | 1756-0500 |
Popis: | Background Optimization of the unsaturated fatty acid composition of ruminant milk and meat is desirable. Alteration of the milk and fatty acid profile was previously attempted by the management of ruminal microbial biohydrogenation. The aim of this study was to identify the group of ruminal trans-vaccenic acid (trans-11 C18:1, t-VA) hydrogenating bacteria by combining enrichment studies in vitro. Methods The enrichment culture growing on t-VA was obtained by successive transfers in medium containing t-VA. Fatty acids were detected by gas chromatograph and changes in the microbial composition during enrichment were analyzed by denaturing gradient gel electrophoresis (DGGE). Prominent DGGE bands of the enrichment cultures were identified by 16S rRNA gene sequencing. Results The growth of ruminal t-VA hydrogenating bacteria was monitored through the process of culture transfer according to the accumulation of stearic acid (C18:0, SA) and ratio of the substrate (t-VA) transformed to the product (SA). A significant part of the retrieved 16S rRNA gene sequences was most similar to those of uncultured bacteria. Bacteria corresponding to predominant DGGE bands in t-VA enrichment cultures clustered with t-VA biohydrogenated bacteria within Group B. Conclusions This study provides more insight into the pathway of biohydrogenation. It also may be important to control the production of t-VA, which has metabolic and physiological benefits, through management of ruminal biohydrogenation bacterium. |
Databáze: | OpenAIRE |
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