Tunicamycin induced endoplasmic reticulum stress in the small intestine
| Autor: | Zübeyde Öztel, Erdal Balcan, Sibel Gazan |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Leukocyte migration Histology Immunofluorescence 03 medical and health sciences chemistry.chemical_compound Peyer's Patches 0302 clinical medicine Intestine Small medicine Intestinal Mucosa 030102 biochemistry & molecular biology medicine.diagnostic_test Endoplasmic reticulum Tunicamycin General Medicine Endoplasmic Reticulum Stress Molecular biology Small intestine Medical Laboratory Technology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis embryonic structures cardiovascular system Immunohistochemistry Reticulum Immunostaining |
| Zdroj: | Biotechnichistochemistry : official publication of the Biological Stain Commission. 96(7) |
| ISSN: | 1473-7760 |
| Popis: | Because the small intestine is exposed to variety of foreign substances, it participates in host immune response. We investigated whether the expression levels of intestinal MAdCAM-1, PECAM-1 (CD31) and CAV-1 are affected by endoplasmic reticulum (ER) stress following brief treatment with tunicamycin (TN). We administered a single dose of TN intraperitoneally. Twenty-four hours later, MAdCAM-1, PECAM-1 and CAV-1 expression levels in Peyer's patches and villi were examined using immunohistochemistry (IHC), immunofluorescence (IF) and western blotting. Immunostaining of MAdCAM-1 and CAV-1 in control and TN treated Peyer's patches and villi exhibited similar staining patterns. The immunoreactivity of PECAM-1 was similar for the control and TN treated Payer's patches, whereas staining was decreased significantly in TN treated villi. Our findings suggest that short term TN treatment did not affect leukocyte movement to lymphoid compartments of the small intestine, but it altered villus architecture due to decreased PECAM-1 expression. |
| Databáze: | OpenAIRE |
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