Genome Sequences and Characterization of the Related Gordonia Phages GTE5 and GRU1 and Their Use as Potential Biocontrol Agents

Autor: Daniel Tillett, Robert J. Seviour, Steve Petrovski
Rok vydání: 2012
Předmět:
Zdroj: Applied and Environmental Microbiology. 78:42-47
ISSN: 1098-5336
0099-2240
Popis: Activated sludge plants suffer frequently from the operational problem of stable foam formation on aerobic reactor surfaces, which can be difficult to prevent. Many foams are stabilized by mycolic acid-containing Actinobacteria , the mycolata. The in situ biocontrol of foaming using phages is an attractive strategy. We describe two polyvalent phages, GTE5 and GRU1, targeting Gordonia terrae and Gordonia rubrupertincta , respectively, isolated from activated sludge. Phage GRU1 also propagates on Nocardia nova . Both phages belong to the family Siphoviridae and have similar-size icosahedral heads that encapsulate double-stranded DNA genomes (∼65 kb). Their genome sequences are similar to each other but markedly different from those of other sequenced phages. Both are arranged in a modular fashion. These phages can reduce or eliminate foam formation by their host cells under laboratory conditions.
Databáze: OpenAIRE