Performance of Human Mass Balance/Metabolite Identification Studies Using Stable Isotope (13C,15N) Labeling and Continuous-Flow Isotope-Ratio Mass Spectrometry as an Alternative to Radioactive Labeling Methods
| Autor: | George K. Szabo, Thomas R. Browne, David A. Wagner, Alfred Ajami |
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| Rok vydání: | 1993 |
| Předmět: |
Pharmacology
Carbon Isotopes Chromatography Nitrogen Isotopes Chemistry Stable isotope ratio Metabolite Urine Mass spectrometry High-performance liquid chromatography Mass Spectrometry Standard curve chemistry.chemical_compound Pharmaceutical Preparations Pharmacokinetics Isotope Labeling Phenobarbital Phenytoin Humans Pharmacology (medical) Isotope-ratio mass spectrometry Chromatography High Pressure Liquid |
| Zdroj: | The Journal of Clinical Pharmacology. 33:246-252 |
| ISSN: | 0091-2700 |
| DOI: | 10.1002/j.1552-4604.1993.tb03951.x |
| Popis: | Stable isotope labeling in therapeutic and subtherapeutic quantities of drug (15N2(13)C-phenobarbital) can be quantitated in biological matrices (urine) and high performance liquid chromatography (HPLC) peaks from urine using continuous-flow isotope-ratio mass spectrometry (CF-IRMS). Standard curves for 15N2(13)C-phenobarbital were reproducible and linear (R2 > 0.985) over the ranges of 3-100 micrograms/ml for whole urine (15N2 or 13C labeling) and 0.1-8.0 micrograms/mL for HPLC peaks derived from urine (15N2 labeling). The lower limit of quantitation values for urine drug concentration was 0.46-2.62 micrograms/mL in whole urine and 0.10-0.70 micrograms/mL in HPLC peaks. Validation samples quantitated with these standard curves yielded close to expected values. These data suggest stable isotope labeling and CF-IRMS may be used as an alternative to 14C labeling and radioactivity counting methods in mass balance/metabolite identification and other biomedical studies. |
| Databáze: | OpenAIRE |
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