Preventive Effect of Curcumin and Quercetin against Nitric Oxide Mediated Modification of Goat Lung Cystatin
| Autor: | Medha Priyadarshini, Mohd Shahnawaz Khan, Bilqees Bano |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Nitroprusside
Curcumin Protein Conformation Flavonoid Nitric Oxide Antioxidants Cysteine Proteinase Inhibitors Nitric oxide chemistry.chemical_compound medicine Animals Lung chemistry.chemical_classification Goats General Chemistry Cystatins carbohydrates (lipids) Papain chemistry Biochemistry Quercetin Cystatin Sodium nitroprusside General Agricultural and Biological Sciences medicine.drug |
| Zdroj: | Journal of Agricultural and Food Chemistry. 57:6055-6059 |
| ISSN: | 1520-5118 0021-8561 |
| Popis: | Cysteine proteinase inhibitors are of prime physiologic importance inside the cells, controlling the activities of lysosomal cysteine proteases. The present work aimed to realize the effects of nitric oxide on the structure and function of goat lung cystatin (GLC) and to evaluate antinitrostative efficacy of curcumin and quercetin. Nitric oxide induced structural modifications were followed by fluorescence spectroscopy and PAGE and functional inactivation by monitoring the inhibition of caseinolytic activity of papain. Ten millimolar sodium nitroprusside (SNP) caused time dependent inactivation of GLC-I with complete functional loss precipitating at 180 min. Curcumin (50 microM) and quercetin (250 microM) opposed such loss in papain inhibitory activity of GLC-I. Loss in tertiary structure of GLC-I (fluorescence quenching and 15 nm red shift) was observed on SNP treatment. Inhibition of functional and structural SNP mediated damage of GLC-I by curcumin (50 microM) and quercetin (250 microM) reaffirms their NO scavenging potency. |
| Databáze: | OpenAIRE |
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