Clonal Vγ6(+)Vδ4(+) T cells promote IL-17–mediated immunity against Staphylococcus aureus skin infection
| Autor: | Xinzhong Dong, Shuting S. Cai, Mark C. Marchitto, Roger V. Ortines, Alina I. Marusina, Robert J. Miller, Wei Shen, Carly A. Dillen, Martin P. Alphonse, Haiyun Liu, Scott I. Simon, Michael R. Yeaman, Alexander A. Merleev, Rebecca L. O'Brien, Scott K. Durum, Isabelle D. Brown, Advaitaa Ravipati, Angel S. Byrd, Lloyd S. Miller, Nathachit Limjunyawong, Emanual Michael Maverakis, Bret L. Pinsker, Emily Zhang, Yu Wang, Nathan K. Archer |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
skin
Staphylococcus aureus T cell T-Lymphocytes T cells Skin infection Biology medicine.disease_cause Proinflammatory cytokine Vaccine Related Mice neutrophils Immunity T-Lymphocyte Subsets medicine 2.1 Biological and endogenous factors Animals Humans Aetiology Multidisciplinary Animal Interleukin-17 Staphylococcal Infections medicine.disease IL-17 Disease Models Animal medicine.anatomical_structure Infectious Diseases Emerging Infectious Diseases PNAS Plus Immunology Disease Models Tumor necrosis factor alpha Immunization Interleukin 17 Lymph Lymph Nodes Infection |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America, vol 116, iss 22 |
| Popis: | T cell cytokines contribute to immunity against Staphylococcus aureus , but the predominant T cell subsets involved are unclear. In an S. aureus skin infection mouse model, we found that the IL-17 response was mediated by γδ T cells, which trafficked from lymph nodes to the infected skin to induce neutrophil recruitment, proinflammatory cytokines IL-1α, IL-1β, and TNF, and host defense peptides. RNA-seq for TRG and TRD sequences in lymph nodes and skin revealed a single clonotypic expansion of the encoded complementarity-determining region 3 amino acid sequence, which could be generated by canonical nucleotide sequences of TRGV5 or TRGV6 and TRDV4 . However, only TRGV6 and TRDV4 but not TRGV5 sequences expanded. Finally, Vγ6 + T cells were a predominant γδ T cell subset that produced IL-17A as well as IL-22, TNF, and IFNγ, indicating a broad and substantial role for clonal Vγ6 + Vδ4 + T cells in immunity against S. aureus skin infections. |
| Databáze: | OpenAIRE |
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