PCR-based determination of Rhc and RhE status of fetuses at risk of Rhc and RhE haemolytic disease
| Autor: | Jean-Pierre Cartron, Y. Brossard, Jacques Chavinie, I Mouro, Yves Colin, Caroline Le Van Kim |
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| Rok vydání: | 1994 |
| Předmět: |
Fetus
Rh-Hr Blood-Group System Base Sequence Red Cell business.industry Molecular Sequence Data Infant Newborn DNA Hematology Amniotic Fluid Rh Isoimmunization Polymerase Chain Reaction law.invention Erythroblastosis Fetal Isoantibodies Risk Factors law Prenatal Diagnosis Immunology Humans Medicine Allele business Polymerase chain reaction Haemolytic disease |
| Zdroj: | British Journal of Haematology. 88:193-195 |
| ISSN: | 1365-2141 0007-1048 |
| DOI: | 10.1111/j.1365-2141.1994.tb04996.x |
| Popis: | Summary. After anti-RhD, anti-Rhc is the most important red cell alloantibody which can cause haemolytic disease of the newborn (HDN) when the mother is Rhc-negative and the fetus Rhc-positive. We report here the development of polymerase chain reaction (PCR) assays which detect the presence of the Rhc alleles in amniotic cells by the use of allele-specific primers (ASP). It is expected that such determination will help in the management of pregnancies at risk of Rhc haemolytic disease. In the course of this study we have similarly performed PCR-ASP experiments to detect fetal RHE alleles since, in rare cases, anti-RhE can also cause HDN. |
| Databáze: | OpenAIRE |
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