smi-miR396b targeted SmGRFs, SmHDT1, and SmMYB37/4 synergistically regulates cell growth and active ingredient accumulation in Salvia miltiorrhiza hairy roots

Autor: Hang Li, Jinjia Zhang, Ronghui Tan, Xiaoyu Zheng, Shujuan Zhao, Min Chen, Zhengtao Wang
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
Transcription
Genetic
Propanols
RNA Stability
Secondary Metabolism
Salvia miltiorrhiza
Plant Science
Acetates
Plant Roots
Salt Stress
01 natural sciences
Anthocyanins
Transcriptome
chemistry.chemical_compound
Gene Expression Regulation
Plant
Gene Regulatory Networks
Biomass
Abscisic acid
Phylogeny
Plant Proteins
Methyl jasmonate
General Medicine
Plants
Genetically Modified
Droughts
Biochemistry
Gibberellin
Cyclopentanes
Alkenes
Biology
03 medical and health sciences
Biosynthesis
Oxylipins
Secondary metabolism
Cell Proliferation
Binding Sites
Terpenes
Cell growth
Gene Expression Profiling
Polyphenols
Gibberellins
MicroRNAs
Gene Ontology
030104 developmental biology
chemistry
Abietanes
Agronomy and Crop Science
Abscisic Acid
Transcription Factors
010606 plant biology & botany
Zdroj: Plant Cell Reports. 39:1263-1283
ISSN: 1432-203X
0721-7714
Popis: MIR396b had been cloned and overexpressed in Salvia miltiorrhiza hairy roots. MiR396b targets SmGRFs, SmHDT1, and SmMYB37/4 to regulate cell growth and secondary metabolism in S. miltiorrhiza hairy roots. Danshen (Salvia miltiorrhiza Bunge) is a valuable medicinal herb with two kinds of clinically used natural products, salvianolic acids and tanshinones. miR396 is a conserved microRNA and plays extensive roles in plants. However, it is still unclear how miR396 works in S. miltiorrhiza. In this study, an smi-MIR396b has been cloned from S. miltiorrhiza. Overexpression of miR396b in danshen hairy roots inhibited hairy root growth, reduced salvianolic acid concentration, but enhanced tanshinone accumulation, resulting in the biomass and total salvianolic acids respectively reduced to 55.5 and 72.1% of the control and total tanshinones increased up to 1.91-fold of the control. Applied degradome sequencing, 5′RLM-RACE, and qRT-PCR, 13 targets for miR396b were identified including seven conserved SmGRF1-7 and six novel ones. Comparative transcriptomics and microRNomics analysis together with qRT-PCR results confirmed that miR396b targets SmGRFs, SmHDT1, and SmMYB37/4 to mediate the phytohormone, especially gibberellin signaling pathways and consequentially resulted in the phenotype variation of miR396b-OE hairy roots. Furthermore, miR396b could be activated by methyl jasmonate, abscisic acid, gibberellin, salt, and drought stresses. The findings in this study indicated that smi-miR396b acts as an upstream and central regulator in cell growth and the biosynthesis of tanshinones and salvianolic acids, shedding light on the coordinated regulation of plant growth and biosynthesis of active ingredients in S. miltiorrhiza.
Databáze: OpenAIRE
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