Placental Barrier to Human Insulin-I125in Insulin-Dependent Diabetic Mothers*
| Autor: | Robert Schwartz, Satish C. Kalhan, Peter A. J. Adam |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Umbilical Veins
medicine.medical_specialty Insulin Antibodies Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry Pregnancy in Diabetics Biochemistry Umbilical vein Placental Membrane Iodine Radioisotopes Endocrinology Pregnancy Diabetes mellitus Internal medicine Placenta medicine Humans Insulin Vein Maternal-Fetal Exchange Fetus business.industry Biochemistry (medical) Venous Plasma medicine.disease Precipitin Tests Blood medicine.anatomical_structure Female business Protein Binding |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 40:139-142 |
| ISSN: | 1945-7197 0021-972X |
| Popis: | Although the normal human placenta is impermeable to insulin, the effect of anti-insulin antibodies on this placental barrier in insulin-dependent diabetic mothers is not known. The placental transfer of human insulin-I125 was examined in 4 normal and 4 insulin-dependent diabetic mothers. Human insulin-I125 was infused at a constant rate for 60–90 min prior to delivery and immuno-precipitable insulin-I125 was measured in the maternal and umbilical venous plasma at delivery. In diabetic patients, insulin-I125 was separated from circulating endogenous anti-insulin antibodiesby acid alcohol extraction prior to immunoprecipita-tion. In normal mothers insulin-I125 radioactivitywas 683 ±110 cpm/ml (mean ± sem) in the maternal vein and 6 ± 2 cpm/ml in the umbilical vein. In three diabetic mothers with high plasma insulin binding capacities between 2580–8400 μU/ml, the umbilical venous insulin-I125 was 10 ± 10 cpm/ml when the maternal plasma insulin-I125 was maintained at 1388 ± 356 cpm/ml even though the antibod... |
| Databáze: | OpenAIRE |
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