The optimal combination of Nile red identification, colony polymerase chain reaction, and gas chromatography detection methods in screening for polyhydroxyalkanoicate-producing bacteria

Autor:	Hung-Che Chou, Chia-Hsin Chen, Hsiao-Kai Chu, Chun-Mei Huang, Hui-Jun Wang, Wei-Lin Tu, Gia-Luen Guo
Rok vydání:	2021
Předmět:	Chromatography Gas Bacteria Polyhydroxyalkanoates Oxazines Genetics General Medicine Molecular Biology Biochemistry Microbiology Polymerase Chain Reaction
Zdroj:	Archives of microbiology. 204(6)
ISSN:	1432-072X
Popis:	The study devised a detection process combining Nile red-containing media, polymerase chain reaction (PCR), and gas chromatography (GC) to evaluate the possibility of microbes becoming polyhydroxyalkanoate (PHA) producers. The Nile red and PCR detection steps of designating PHA producers had true positive rates of 39.4% and 40%, respectively, and the use of GC analysis as the final step yielded accurate results for the production types and yields of PHAs. When the number of screening samples was up to 10
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3df1e28259b08d5fdbc841b978f91d3e https://pubmed.ncbi.nlm.nih.gov/35538332 Zobrazit plný text záznamu Full text from SpringerLink