Effects of storage time on Cytomegalovirus DNA stability in plasma determined by quantitative real-time PCR
| Autor: | Jian Wang, Taijie Li, Yan Deng, Shan Huang, Yu He, Li Xie, Xue Qin, Xiao-Nan Liang, Shan Li |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Time Factors Adolescent medicine.medical_treatment Congenital cytomegalovirus infection Cytomegalovirus Hematopoietic stem cell transplantation Biology Real-Time Polymerase Chain Reaction Edta plasma Cytomegalovirus DNA Specimen Handling chemistry.chemical_compound Plasma Young Adult Virology medicine Humans Child Reproducibility Temperature Middle Aged Viral Load medicine.disease Transplant Recipients Quantitative Real Time PCR chemistry Child Preschool Cytomegalovirus Infections DNA Viral Female Viral load DNA |
| Zdroj: | Journal of virological methods. 207 |
| ISSN: | 1879-0984 |
| Popis: | Quantitative real-time PCR (QRT-PCR) assays are faster, more precise, and more sensitive quantitative laboratory methods for monitoring serial CMV DNA viral load in patients undergoing organ or hematopoietic stem cell transplantation. Clinical laboratories often face practical concerns about the storage of specimens from these patients to ensure the accuracy and reproducibility of CMV viral load test results. Different studies that have assessed CMV DNA stability have shown mixed results. Therefore, we analyzed CMV DNA stability of 30 EDTA plasma samples in samples containing between 300 and 100,000copies/ml over a 21 day period. The concentration of CMV DNA in all samples stored at 4°C for 21 days did not differ significantly from the baseline viral load (t=0.242, p=0.810), and no trend was evident to indicate continued degradation over a 2 week period. |
| Databáze: | OpenAIRE |
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