Environmental control of the cell cycle in Drosophila: nutrition activates mitotic and endoreplicative cells by distinct mechanisms
| Autor: | Bruce A. Edgar, Jessica S. Britton |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
DNA Replication
Cell type animal structures Fat Body Cell Cycle Proteins Environment Biology Models Biological Neuroblast Culture Techniques Animals Drosophila Proteins Endoreduplication Amino Acids E2F Molecular Biology Mitosis Cells Cultured Glycoproteins Neurons Stem Cells Cell Cycle fungi Cell cycle Cell biology Imaginal disc Larva Insect Proteins Animal Nutritional Physiological Phenomena Drosophila Mitogens Stem cell Signal Transduction Developmental Biology |
| Zdroj: | Development. 125:2149-2158 |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.125.11.2149 |
| Popis: | In newly hatched Drosophila larvae, quiescent cells reenter the cell cycle in response to dietary amino acids. To understand this process, we varied larval nutrition and monitored effects on cell cycle initiation and maintenance in the mitotic neuroblasts and imaginal disc cells, as well as the endoreplicating cells in other larval tissues. After cell cycle activation, mitotic and endoreplicating cells respond differently to the withdrawal of nutrition: mitotic cells continue to proliferate in a nutrition-independent manner, while most endoreplicating cells reenter a quiescent state. We also show that ectopic expression of Drosophila Cyclin E or the E2F transcription factor can drive quiescent endoreplicating cells, but not quiescent imaginal neuroblasts, into S-phase. Conversely, we demonstrate that quiescent imaginal neuroblasts, but not quiescent endoreplicating cells, can be induced to enter the cell cycle when co-cultured with larval fat body in vitro. These results demonstrate a fundamental difference in the control of cell cycle activation and maintenance in these two cell types, and imply the existence of a novel mitogen generated by the larval fat body in response to nutrition. |
| Databáze: | OpenAIRE |
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