Effects of Overproduction of Rubisco Activase on Rubisco Content in Transgenic Rice Grown at Different N Levels

Autor: Yuji Suzuki, Amane Makino, Mao Suganami, So Konno, Shinji Nishida, Eri Kondo
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
Rubisco Small Subunit
inorganic chemicals
Ribulose-Bisphosphate Carboxylase
Photosynthesis
01 natural sciences
Article
Catalysis
nitrogen
Inorganic Chemistry
lcsh:Chemistry
03 medical and health sciences
Gene Expression Regulation
Plant
Physical and Theoretical Chemistry
Promoter Regions
Genetic
Overproduction
Molecular Biology
lcsh:QH301-705.5
Cells
Cultured
Spectroscopy
Plant Proteins
photosynthesis improvement
Oryza sativa
biology
Chemistry
Organic Chemistry
RuBisCO
fungi
Rubisco
Rubisco activase
Rubisco activation
photosynthesis improvement
nitrogen
transgenic rice
food and beverages
Oryza
General Medicine
Carbon Dioxide
Plants
Genetically Modified
Genetically modified rice
Computer Science Applications
rubisco activase
Plant Leaves
Horticulture
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
rubisco activation
transgenic rice
biology.protein
rubisco
010606 plant biology & botany
Zdroj: International Journal of Molecular Sciences, Vol 21, Iss 5, p 1626 (2020)
International Journal of Molecular Sciences
Volume 21
Issue 5
ISSN: 1422-0067
Popis: It has been reported that overproduction of Rubisco activase (RCA) in rice (Oryza sativa L.) decreased Rubisco content, resulting in declining photosynthesis. We examined the effects of RCA levels on Rubisco content using transgenic rice with overexpressed or suppressed RCA under the control of different promoters of the RCA and Rubisco small subunit (RBCS) genes. All plants were grown hydroponically with different N concentrations (0.5, 2.0 and 8.0 mM-N). In RCA overproduced plants with >
2-fold RCA content (RCA-HI lines), a 10%&ndash
20% decrease in Rubisco content was observed at 0.5 and 2.0 mM-N. In contrast, at 8.0 mM-N, Rubisco content did not change in RCA-HI lines. Conversely, in plants with 50%&ndash
60% increased RCA content (RCA-MI lines), Rubisco levels remained unchanged, regardless of N concentration. Such effects on Rubisco content were independent of the promoter that was used. In plants with RCA suppression to <
10% of the wild-type RCA content, Rubisco levels were increased at 0.5 mM-N, but were unchanged at 2.0 and 8.0 mM-N. Thus, the effects of the changes in RCA levels on Rubisco content depended on N supply. Moreover, RCA overproduction was feasible without a decrease in Rubisco content, depending on the degree of RCA production.
Databáze: OpenAIRE
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