Effects of Overproduction of Rubisco Activase on Rubisco Content in Transgenic Rice Grown at Different N Levels
Autor: | Yuji Suzuki, Amane Makino, Mao Suganami, So Konno, Shinji Nishida, Eri Kondo |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
0301 basic medicine Rubisco Small Subunit inorganic chemicals Ribulose-Bisphosphate Carboxylase Photosynthesis 01 natural sciences Article Catalysis nitrogen Inorganic Chemistry lcsh:Chemistry 03 medical and health sciences Gene Expression Regulation Plant Physical and Theoretical Chemistry Promoter Regions Genetic Overproduction Molecular Biology lcsh:QH301-705.5 Cells Cultured Spectroscopy Plant Proteins photosynthesis improvement Oryza sativa biology Chemistry Organic Chemistry RuBisCO fungi Rubisco Rubisco activase Rubisco activation photosynthesis improvement nitrogen transgenic rice food and beverages Oryza General Medicine Carbon Dioxide Plants Genetically Modified Genetically modified rice Computer Science Applications rubisco activase Plant Leaves Horticulture 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 rubisco activation transgenic rice biology.protein rubisco 010606 plant biology & botany |
Zdroj: | International Journal of Molecular Sciences, Vol 21, Iss 5, p 1626 (2020) International Journal of Molecular Sciences Volume 21 Issue 5 |
ISSN: | 1422-0067 |
Popis: | It has been reported that overproduction of Rubisco activase (RCA) in rice (Oryza sativa L.) decreased Rubisco content, resulting in declining photosynthesis. We examined the effects of RCA levels on Rubisco content using transgenic rice with overexpressed or suppressed RCA under the control of different promoters of the RCA and Rubisco small subunit (RBCS) genes. All plants were grown hydroponically with different N concentrations (0.5, 2.0 and 8.0 mM-N). In RCA overproduced plants with > 2-fold RCA content (RCA-HI lines), a 10%&ndash 20% decrease in Rubisco content was observed at 0.5 and 2.0 mM-N. In contrast, at 8.0 mM-N, Rubisco content did not change in RCA-HI lines. Conversely, in plants with 50%&ndash 60% increased RCA content (RCA-MI lines), Rubisco levels remained unchanged, regardless of N concentration. Such effects on Rubisco content were independent of the promoter that was used. In plants with RCA suppression to < 10% of the wild-type RCA content, Rubisco levels were increased at 0.5 mM-N, but were unchanged at 2.0 and 8.0 mM-N. Thus, the effects of the changes in RCA levels on Rubisco content depended on N supply. Moreover, RCA overproduction was feasible without a decrease in Rubisco content, depending on the degree of RCA production. |
Databáze: | OpenAIRE |
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