Integrated Transcriptome and Proteome Studies Reveal the Underlying Mechanisms for Sterol Catabolism and Steroid Production in Mycobacterium neoaurum
| Autor: | Min Liu, Xinyi Tao, Dongzhi Wei, Qinghai Liu, Feng-Qing Wang, Liang-Bin Xiong |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Proteome medicine.medical_treatment 030106 microbiology Mutant Regulon Mycobacterium Steroid Transcriptome 03 medical and health sciences Bacterial Proteins Biotransformation Mycobacterium neoaurum medicine biology Chemistry Gene Expression Regulation Bacterial General Chemistry biology.organism_classification Sterol Sterols 030104 developmental biology Biochemistry Multigene Family Steroids General Agricultural and Biological Sciences |
| Zdroj: | Journal of Agricultural and Food Chemistry. 66:9147-9157 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.8b02714 |
| Popis: | Integrated transcriptome and proteome studies were performed to investigate sterol biotransformation in wild-type Mycobacterium neoaurum ATCC 25795 ( Mn) and the mutant strains producing steroid intermediates. Transcriptome and proteome studies indicated that several metabolic activities were noticeably dynamic, including cholesterol degradation, central carbon metabolism, cell envelope biosynthesis, glycerol metabolism, and transport. Interestingly, a poor overall correlation between mRNA and translation profiles was found, which might contribute to the metabolic adaptation in cholesterol catabolism. A gene cluster covering 111 genes was discovered to encode for cholesterol catabolism in Mn. Generally, transcription and/or translation of the genes in KstR1 regulon was upregulated, and the induction of genes in KstR2 regulon was not as significant as that of KstR1 regulon. Several induced genes showing potential roles for cholesterol catabolism were found. Further identification of these genes and investigation of the correlation among key metabolic activities could help for the development of efficient steroid-producing strains. |
| Databáze: | OpenAIRE |
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