Genetic Toolkits to Design and Build Mammalian Synthetic Systems
| Autor: | Tom Ellis, Roberto Di Blasi, Annalise Zouein, Francesca Ceroni |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Orthogonality (programming) Computer science Genetic Vectors cloning standardisation Bioengineering 02 engineering and technology Computational biology DNA construct 09 Engineering 03 medical and health sciences Synthetic biology Chromosome (genetic algorithm) 10 Technology Animals Golden gate Cloning Molecular Cloning (programming) business.industry DNA 06 Biological Sciences Modular design 021001 nanoscience & nanotechnology Key features mammalian synthetic biology 030104 developmental biology design–build–test cycle Synthetic Biology Genetic Engineering 0210 nano-technology business human activities Biotechnology |
| Zdroj: | Trends in Biotechnology. 39:1004-1018 |
| ISSN: | 0167-7799 |
| Popis: | Construction of DNA-encoded programs is central to synthetic biology and the chosen method often determines the time required to design and build constructs for testing. Here we describe and summarise key features of the available toolkits for DNA construction for mammalian cells. We compare the different cloning strategies based on their complexity and the time needed to generate constructs of different sizes, and we reflect on why Golden Gate toolkits now dominate due to their modular design. We look forward to future advances, including accessory packs to cloning toolkits that can facilitating editing, orthogonality, advanced regulation, and integration into synthetic chromosome construction. |
| Databáze: | OpenAIRE |
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