Monoclonal Antibodies for Dengue Virus prM Glycoprotein Protect Mice against Lethal Dengue Infection
| Autor: | R.L. Timchak, P. L. Summers, Doria R. Dubois, W. Houston Cohen, Donald S. Burke, Kenneth H. Eckels, Bennett M. Kaufman, Mary K. Gentry |
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| Rok vydání: | 1989 |
| Předmět: |
medicine.drug_class
viruses Blotting Western Heterologous Enzyme-Linked Immunosorbent Assay Dengue virus Antibodies Viral Monoclonal antibody medicine.disease_cause Virus Dengue fever Dengue Neutralization Tests Virology medicine Humans Antigens Viral Glycoproteins Viral Structural Proteins biology Immunization Passive Antibodies Monoclonal virus diseases Dengue Virus medicine.disease Complement fixation test biology.organism_classification humanities Flavivirus Infectious Diseases biology.protein Parasitology Antibody |
| Zdroj: | The American Journal of Tropical Medicine and Hygiene. 41:576-580 |
| ISSN: | 1476-1645 0002-9637 |
| Popis: | Five murine monoclonal antibodies (Mabs) reactive against the prM glycoproteins of DEN-3 and -4 were used to passively protect mice in vivo against lethal challenge with homologous and heterologous dengue virus serotypes. Four of the 5 prM-reactive monoclonals cross-protected mice against heterologous challenge, whereas 1 protected against challenge with only the homologous serotype. Although in vitro binding to virions was readily demonstrated, only 2 of the prM Mabs had detectable neutralizing activity. The neutralizing activity could not be enhanced by anti-mouse immunoglobulin or complement. However, 4 of the 5 prM Mabs fixed complement. This is the first report of prM-specific Mabs that are protective in mice. |
| Databáze: | OpenAIRE |
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