Design of a red-emitter hybrid material for bioimaging: europium complexes grafted on silica particles
| Autor: | A.M.G. Mutti, Aldo Eloizo Job, G.R. Teixeira, Ana Maria Pires, Dalita G. S. M. Cavalcante, S. A. M. Lima, Andressa S. Gomes, J.A.O. Santos |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Materials science
Polymers and Plastics Biocompatibility chemistry.chemical_element Nanoparticle 02 engineering and technology 010402 general chemistry 01 natural sciences Catalysis Biomaterials Colloid and Surface Chemistry Microscopy Lanthanides Materials Chemistry Zeta potential Inorganic-organic hybrids Functionalization Photoluminescence 021001 nanoscience & nanotechnology Fluorescence 0104 chemical sciences Electronic Optical and Magnetic Materials chemistry 0210 nano-technology Hybrid material Luminescence Europium Biomarkers Nuclear chemistry |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2020-12-12T01:05:49Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-12-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) A bottom-up approach was used to design a high luminescent biomarker composed of nanoparticles of silica grafted with Eu3+ complex, and the labeling of CHO-k1 ovary cells and gastrocnemius muscle cells was successfully observed in imaging tests. Spheroidal silica nanoparticles were prepared via the solgel method and then decorated with Eu3+ complexes in a sequence of reactions to form the final hybrid nanoparticles. The material was characterized by scanning electron microscopy (120 ± 19 nm), zeta potential (−15 mV), and photoluminescence (red emission with 32% quantum efficiency). The material exhibit high biocompatibility with CHO-k1 cells, as evidenced via the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method. CHO-k1 cells cultivated in the presence of the hybrid were investigated by microscopy techniques, and an active mechanism of internalization via phagocytosis was evidenced. Fluorescence and confocal images indicated red emission distributed in the cytoplasm, and especially around the nuclei. While for the muscle cells also stained with the luminescent particles, a distribution near the membrane was observed. Thus, the designed system proved to be safe and appropriate for use in bioimaging assays. São Paulo State University (Unesp) School of Technology and Sciences São Paulo State University (Unesp) Institute of Biosciences Humanities and Exact Sciences São Paulo State University (Unesp) School of Technology and Sciences São Paulo State University (Unesp) Institute of Biosciences Humanities and Exact Sciences FAPESP: 2018/13538–2 |
| Databáze: | OpenAIRE |
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