The Higher Structure of Chromatin in the LCR of the β-Globin Locus Changes during Development
| Autor: | George Stamatoyannopoulos, Ping Xiang, Qiliang Li, Xiangdong Fang, Wenxuan Yin, Hemei Han |
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| Rok vydání: | 2009 |
| Předmět: |
Erythroblasts
Embryonic Development Mice Transgenic beta-Globins Biology urologic and male genital diseases Protein Structure Secondary Article Histones Chromosome conformation capture Mice Structural Biology hemic and lymphatic diseases Animals Deoxyribonuclease I Humans Micrococcal Nuclease Molecular Biology ChIA-PET Locus control region Gene Expression Regulation Developmental Acetylation Embryo Mammalian Locus Control Region Molecular biology Chromatin Nucleosomes Mutation embryonic structures DNase I hypersensitive site Chromatin immunoprecipitation Hypersensitive site |
| Zdroj: | Journal of Molecular Biology. 394:197-208 |
| ISSN: | 0022-2836 |
| Popis: | The beta-globin locus control region (LCR) is able to enhance the expression of all globin genes throughout the course of development. However, the chromatin structure of the LCR at the different developmental stages is not well defined. We report DNase I and micrococcal nuclease hypersensitivity, chromatin immunoprecipitation analyses for histones H2A, H2B, H3, and H4, and 3C (chromatin conformation capture) assays of the normal and mutant beta-globin loci, which demonstrate that nucleosomes at the DNase I hypersensitive sites of the LCR could be either depleted or retained depending on the stages of development. Furthermore, MNase sensitivity and 3C assays suggest that the LCR chromatin is more open in embryonic erythroblasts than in definitive erythroblasts at the primary- and secondary-structure levels; however, the LCR chromatin is packaged more tightly in embryonic erythroblasts than in definitive erythroblasts at the tertiary chromatin level. Our study provides the first evidence that the occupancy of nucleosomes at a DNase I hypersensitive site is a developmental stage-related event and that embryonic and adult cells possess distinct chromatin structures of the LCR. |
| Databáze: | OpenAIRE |
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