The cellular level of O-antigen polymerase Wzy determines chain length regulation by WzzB and WzzpHS-2 in Shigella flexneri 2a
| Autor: | Javier A. Carter, Mercedes Zaldívar, Juan Cristobal Jimenez, Cristina L. Marolda, Sergio A. Álvarez, Inés Contreras, Miguel A. Valvano |
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| Rok vydání: | 2009 |
| Předmět: |
Immunoprecipitation
Repeating unit(s) RU DSG Microbiology Article Shigella flexneri Disuccinimidyl glutarate Bacterial Proteins Antigen Western blot medicine VL-OAg Cloning Molecular DSP Polymerase Immunology and Infectious Disease Dithio-bis(succinimidylpropionate) Short O-antigen biology medicine.diagnostic_test S-OAg O Antigens Promoter Gene Expression Regulation Bacterial biology.organism_classification Molecular biology Enterobacteriaceae Hexosyltransferases Membrane protein biology.protein Very long O antigen |
| Zdroj: | Microbiology & Immunology Publications |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/mic.0.028944-0 |
| Popis: | The lipopolysaccharide O antigen of Shigella flexneri 2a has two preferred chain lengths, a short (S-OAg) composed of an average of 17 repeated units and a very long (VL-OAg) of about 90 repeated units. These chain length distributions are controlled by the chromosomally encoded WzzB and the plasmid-encoded WzzpHS-2 proteins, respectively. In this study, genes wzzB, wzz pHS-2 and wzy (encoding the O-antigen polymerase) were cloned under the control of arabinose- and rhamnose-inducible promoters to investigate the effect of varying their relative expression levels on O antigen polysaccharide chain length distribution. Controlled expression of the chain length regulators wzzB and wzz pHS-2 revealed a dose-dependent production of each modal length. Increase in one mode resulted in a parallel decrease in the other, indicating that chain length regulators compete to control the degree of O antigen polymerization. Also, when expression of the wzy gene is low, S-OAg but not VL-OAg is produced. Production of VL-OAg requires high induction levels of wzy. Thus, the level of expression of wzy is critical in determining O antigen modal distribution. Western blot analyses of membrane proteins showed comparable high levels of the WzzB and WzzpHS-2 proteins, but very low levels of Wzy. In vivo cross-linking experiments and immunoprecipitation of membrane proteins did not detect any direct interaction between Wzy and WzzB, suggesting the possibility that these two proteins may not interact physically but rather by other means such as via translocated O antigen precursors. |
| Databáze: | OpenAIRE |
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