Chemical characterization, cytotoxic, antioxidant, antimicrobial, and enzyme inhibitory effects of different extracts from one sage (Salvia ceratophylla L.) from Turkey: open a new window on industrial purposes
| Autor: | Zoltán Cziáky, Luísa Custódio, Fevzi Elbasan, Gokhan Zengin, Ramazan Ceylan, Nabeelah Bibi Sadeer, Kouadio Ibrahime Sinan, Ahmet Uysal, Sengul Uysal, Gunes Ak, Evren Yildiztugay, Maria João Rodrigues, József Jekő, Mohamad Fawzi Mahomoodally |
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| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
ABTS 010405 organic chemistry DPPH General Chemical Engineering Rosmarinic acid Flavonoid General Chemistry 01 natural sciences 0104 chemical sciences 03 medical and health sciences chemistry.chemical_compound Rutin 0302 clinical medicine chemistry Polyphenol 030212 general & internal medicine Gallic acid Food science Kojic acid |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/d0ra10044g |
| Popis: | In the present study, the methanolic, hydro-methanolic, dichloromethane, hexane and aqueous extracts of Salvia ceratophylla L. (Family: Lamiaceae), a lemon-scented herb, were tested for total phenolic (TPC) and flavonoid content (TFC) and antioxidant activities were evaluated using a battery of assays (2,2-diphenyl-1- picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity, total antioxidant capacity (TAC) (phosphomolybdenum) and metal chelating). Enzyme inhibitory effects were investigated using acetyl- (AChE), butyryl-cholinesterase (BChE), tyrosinase, a-amylase and a-glucosidase as target enzymes. Regarding the cytotoxic abilities, HepG2, B164A5 and S17 cell lines were used. The phytochemical profile was conducted using liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). Our data showed that the methanolic aerial extracts possessed the highest phenolic (72.50 0.63 mg gallic acid equivalent per g) and flavonoid (43.77 1.09 mg rutin equivalent per g) contents. The hydromethanolic aerial extract showed significant DPPH radical scavenging activity (193.40 0.27 mg TE per g) and the highest reducing potential against CUPRAC (377.93 2.38 mg TE per g). The best tyrosinase activity was observed with dichloromethane root extract (125.45 1.41 mg kojic acid equivalent per g). Among the tested extracts, hexane root extract exerted the highest antimicrobial potential with a minimum inhibitory concentration value of 0.048 mg mL 1. Methanolic root extract showed the lowest cytotoxicity (28%) against HepG2 cells. Phytochemical analysis revealed the presence of important polyphenolic compounds including luteolin, gallic acid, rosmarinic acid, to name a few. This research can be used as one methodological starting point for further investigations on this lemon-scented herb. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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