Detection and comparison of reactive oxygen species (ROS) generated by chlorophyllin metal (Fe, Mg and Cu) complexes under ultrasonic and visible-light irradiation
| Autor: | Jun Wang, Jingqun Gao, Baoxin Wang, Xudong Jin, Zhiqiu Wang, Ying Li, Kai Li, Yuwei Guo |
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| Rok vydání: | 2011 |
| Předmět: |
Absorption (pharmacology)
Light Acoustics and Ultrasonics medicine.medical_treatment Photodynamic therapy Radiation Dosage Photochemistry Inorganic Chemistry Metal Sonication chemistry.chemical_compound medicine Chemical Engineering (miscellaneous) Environmental Chemistry Radiology Nuclear Medicine and imaging Irradiation chemistry.chemical_classification Reactive oxygen species Chlorophyllides Chlorophyllin Organic Chemistry Sonodynamic therapy chemistry visual_art visual_art.visual_art_medium Carbazide Reactive Oxygen Species |
| Zdroj: | Ultrasonics Sonochemistry. 18:1028-1034 |
| ISSN: | 1350-4177 |
| DOI: | 10.1016/j.ultsonch.2010.12.006 |
| Popis: | In this paper, in order to examine the mechanisms of sonodynamic and photodynamic reactions, the chlorophyllin metal (Chl-M (M=Fe, Mg and Cu)) complexes were irradiated by ultrasound (US) and visible-light (VL), respectively, and the generation of reactive oxygen species (ROS) were detected by the method of Oxidation-Extraction Spectrometry (OES). That is, the 1,5-diphenyl carbazide (DPCI) is oxidized by the generated ROS into 1,5-diphenyl carbazone (DPCO), which can display a various visible absorption around 563 nm wavelength. Besides, some influence parameters on the generation of ROS were also reviewed. The results demonstrated an apparent synergistic effect of Chl-M and ultrasonic or visible-light irradiation for the generation of ROS. Moreover, the quantities of generated ROS increase with the increase of (ultrasonic or visible-light) irradiation time and Chl-M (M=Fe, Mg and Cu) concentration. Finally, several quenchers were used to determine the kind of the generated ROS. It is wished that this paper might offer some valuable references for the study on the sonodynamic therapy (SDT) and photodynamic therapy (PDT) mechanisms and the application of Chl-M in tumor treatment. |
| Databáze: | OpenAIRE |
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