The human cytoplasmic dynein interactome reveals novel activators of motility

Autor: Ian Hollyer, Laurence Florens, William B. Redwine, Zaw Min Htet, Michael P. Washburn, Morgan E. DeSantis, Samara L. Reck-Peterson, Selene K. Swanson, Phuoc Tien Tran
Rok vydání: 2017
Předmět:
Cytoplasmic Dyneins
0301 basic medicine
Microtubules
Interactome
0302 clinical medicine
Cell Movement
Biology (General)
Cytoskeleton
chemistry.chemical_classification
0303 health sciences
dynein
General Neuroscience
cytoskeleton
Dynactin Complex
General Medicine
Tools and Resources
Cell biology
molecular motor
Medicine
single-molecule
microtubule
Human
QH301-705.5
Science
Protein subunit
Cytological Techniques
Dynein
macromolecular substances
Biology
General Biochemistry
Genetics and Molecular Biology
Cell Line
03 medical and health sciences
proteomics
Microtubule
Humans
030304 developmental biology
DNA ligase
Staining and Labeling
General Immunology and Microbiology
Activator (genetics)
Cell Biology
030104 developmental biology
chemistry
Cytoplasm
Dynactin
Carrier Proteins
030217 neurology & neurosurgery
Zdroj: eLife
eLife, Vol 6 (2017)
ISSN: 2050-084X
Popis: In human cells, cytoplasmic dynein-1 is essential for long-distance transport of many cargos, including organelles, RNAs, proteins, and viruses, towards microtubule minus ends. To understand how a single motor achieves cargo specificity, we identified the human dynein interactome or “transportome” by attaching a promiscuous biotin ligase (“BioID”) to seven components of the dynein machinery, including a subunit of the essential cofactor dynactin. This method reported spatial information about the large cytosolic dynein/dynactin complex in living cells. To achieve maximal motile activity and to bind its cargos, human dynein/dynactin requires “activators”, of which only five have been described. We developed methods to identify new activators in our BioID data, and discovered that ninein and ninein-like are a new family of dynein activators. Analysis of the protein interactomes for six activators, including ninein and ninein-like, suggests that each dynein activator has multiple cargos.
Databáze: OpenAIRE
Externí odkaz: