Epidemiology and molecular characterization of a clone of Burkholderia cenocepacia responsible for nosocomial pulmonary tract infections in a French intensive care unit

Autor: Claude Bouvet, Frédéric Laurent, Manuelle Neto, Benoit Cournoyer, Henri de Montclos, Aymeric Menard, Sandrine Gaillard, Roger Miollan, Arnault Graindorge
Přispěvatelé: Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Centre Hospitalier de Bourg-en-Bresse, Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Ecologie microbienne ( EM ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Vétérinaire de Lyon ( ENVL ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -VetAgro Sup ( VAS )
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Male
Burkholderia cenocepacia
[ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
B. cepacia
Polymerase Chain Reaction
Disease Outbreaks
B. vietnamiensis
Genotype
Respiratory Tract Infections
Phylogeny
0303 health sciences
Cross Infection
Molecular Epidemiology
biology
Virulence
Burkholderia multivorans
Burkholderia Infections
General Medicine
Middle Aged
3. Good health
Electrophoresis
Gel
Pulsed-Field
Intensive Care Units
Infectious Diseases
Female
France
Polymorphism
Restriction Fragment Length
Microbiology (medical)
Burkholderia
B. multivorans
Microbial Sensitivity Tests
Cystic fibrosis
Microbiology
03 medical and health sciences
Young Adult
Bacterial Proteins
Intensive care
Drug Resistance
Bacterial
Pulsed-field gel electrophoresis
Humans
030304 developmental biology
Aged
[ SDE.BE ] Environmental Sciences/Biodiversity and Ecology
[SDV.GEN]Life Sciences [q-bio]/Genetics
Molecular epidemiology
030306 microbiology
Nosocomial outbreak
Pneumonia
biology.organism_classification
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Rec A Recombinases
Burkholderia vietnamiensis
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
[ SDV.GEN ] Life Sciences [q-bio]/Genetics
Zdroj: Diagnostic Microbiology and Infectious Disease
Diagnostic Microbiology and Infectious Disease, Elsevier, 2010, pp.29-40. ⟨10.1016/j.diagmicrobio.2009.06.008⟩
Diagnostic Microbiology and Infectious Disease, Elsevier, 2010, pp.29-40. 〈10.1016/j.diagmicrobio.2009.06.008〉
ISSN: 0732-8893
DOI: 10.1016/j.diagmicrobio.2009.06.008⟩
Popis: International audience; Clustered cases of nosocomial pulmonary infections were observed in a French intensive care unit. Biochemical tests showed the etiologic agents to be part of the Bcc (Bcc). recA polymerase chain reaction-restriction fragment length polymorphism analysis and molecular phylogeny positioned the isolates into Burkholderia cenocepacia. Their recA sequences were found identical to those of ET12 strains responsible of necrotic pneumonia in cystic fibrosis patients. Analyses of a multi locus sequence typing genes set confirmed this proximity and suggested a wide distribution among occidental countries but could not resolve their phylogenetic position unambiguously. A novel marker, ecfB, indicated a significant phylogenetic divergence from ET12 strains. Pulse field gel electrophoresis analysis of SpeI-restricted total genomic DNA of the strains showed a unique profile indicative of a clonal outbreak. Environmental hospital screenings indicated crosscontamination between staff and patients. Bcc strains from outdoor environments were not related to this clone but indicated the presence of Burkholderia multivorans and Burkholderia vietnamiensis.
Databáze: OpenAIRE
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