The 2.1-Å crystal structure of an archaeal preinitiation complex: TATA-box-binding protein/transcription factor (II)B core/TATA-box
| Autor: | Brian S. DeDecker, Paul B. Sigler, Gourishankar Ghosh, Peter F. Kosa |
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| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
Models
Molecular Protein Folding Protein Conformation Molecular Sequence Data Arabidopsis Transcription factor complex Biology Crystallography X-Ray Protein Structure Secondary Species Specificity Computer Graphics Humans Amino Acid Sequence Sequence Deletion Genetics Multidisciplinary General transcription factor Base Sequence TATA-Box Binding Protein Eukaryotic transcription DNA Biological Sciences Archaea TATA Box Recombinant Proteins Cell biology Protein Structure Tertiary DNA-Binding Proteins Oligodeoxyribonucleotides Transcription preinitiation complex Transcription Factor TFIIB Nucleic Acid Conformation Transcription factor II E Transcription factor II D Transcription factor II A Transcription Factors |
| Popis: | Archaea possess a basal transcriptional apparatus that resembles that of eukaryotes. Here we report the 2.1-Å crystal structure of the archaeal transcription factor complex formed by the TATA-box-binding protein (TBP), the transcription factor IIB homolog, and a DNA target, all from the hyperthermophile Pyrococcus woesei . The overall fold of these two basal transcription factors is essentially the same as that of their eukaryotic counterparts. However, in comparison with the eukaryotic complexes, the archaeal TBP–DNA interface is more symmetrical, and in this structure the orientation of the preinitiation complex assembly on the promoter is inverted with respect to that seen in all crystal structures of comparable eukaryotic systems. This study of the structural details of an archaeal transcription factor complex presents the opportunity to examine the evolution of the basal eukaryotic transcriptional apparatus from a stereochemical viewpoint and to extend our understanding of the physical biochemistry of transcriptional initiation. |
| Databáze: | OpenAIRE |
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