Nucleotide sequence of the Staphylococcus aureus RNA polymerase rpoB gene and comparison of its predicted amino acid sequence with those of other bacteria
| Autor: | Geoffrey Coleman, Geoffrey C. Rowland, Mohamed Aboshkiwa |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Salmonella typhimurium
Staphylococcus aureus Molecular Sequence Data Biophysics Biology Biochemistry Homology (biology) chemistry.chemical_compound Start codon Structural Biology RNA polymerase Genetics Escherichia coli Amino Acid Sequence Gene Peptide sequence chemistry.chemical_classification Base Sequence Sequence Homology Amino Acid Pseudomonas putida Nucleic acid sequence DNA-Directed RNA Polymerases rpoB Molecular biology Anabaena Amino acid chemistry Genes Bacterial bacteria |
| Zdroj: | Biochimica et biophysica acta. 1262(1) |
| ISSN: | 0006-3002 |
| Popis: | The complete nucleotide sequence of the rpoB gene which encodes the beta subunit of S. aureus RNA polymerase has been determined. The RpoB protein, consists of 1182 amino acids and has a novel initiation codon UUG which initiates protein synthesis with methionine. There is a very strong Shine-Dalgarno complementarity and the -10 and -35 promoter hexameric sequences are TAATAT and CCGTTT, respectively. A rho-dependent termination site, CAATCAA, is present which overlaps the -35 promoter sequence of the adjacent rpoC gene a feature which may have a role in the co-ordinate expression of the two genes. A strong homology and conserved regions were found to exist over the predicted amino acid sequences coding for S. aureus rpoB and the equivalent proteins in Escherichia coli, Pseudomonas putida, Salmonella typhimurium, Chlamydia trachomatis, cyanobacterium Anabaena sp. strain PCC 7120. |
| Databáze: | OpenAIRE |
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