Potential applicability of cytokines as biomarkers of disease activity in rheumatoid arthritis: Enzyme-linked immunosorbent spot assay-based evaluation of TNF-α, IL-1β, IL-10 and IL-17A

Autor: Ajith Sominanda, Chandrika Jayasinghe, Priyani Wanigasekara, Keerthie Dissanayake
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Male
Enzyme-Linked Immunospot Assay
Physiology
medicine.medical_treatment
Interleukin-1beta
Arthritis
Biochemistry
Severity of Illness Index
Arthritis
Rheumatoid
Mathematical and Statistical Techniques
Medical Conditions
0302 clinical medicine
Immune Physiology
Medicine and Health Sciences
Cell Cycle and Cell Division
Enzyme-Linked Immunoassays
Innate Immune System
Multidisciplinary
ELISPOT
Statistics
Interleukin-17
Middle Aged
Body Fluids
Interleukin-10
Blood
Cytokine
Cell Processes
Rheumatoid arthritis
Physical Sciences
Cytokines
Regression Analysis
Biomarker (medicine)
Medicine
Female
Anatomy
Research Article
Adult
medicine.medical_specialty
Inflammatory Diseases
Science
Immunology
Rheumatoid Arthritis
Linear Regression Analysis
Research and Analysis Methods
Peripheral blood mononuclear cell
Autoimmune Diseases
03 medical and health sciences
Rheumatology
Internal medicine
medicine
Humans
Statistical Methods
Immunoassays
030203 arthritis & rheumatology
Tumor Necrosis Factor-alpha
business.industry
Biology and Life Sciences
Cell Biology
Molecular Development
medicine.disease
Immune System
Immunologic Techniques
Clinical Immunology
IL17A
Clinical Medicine
Mitogens
business
Biomarkers
Mathematics
Developmental Biology
030215 immunology
Zdroj: PLoS ONE, Vol 16, Iss 1, p e0246111 (2021)
PLoS ONE
ISSN: 1932-6203
Popis: Biomarkers play a pivotal role in the management of rheumatoid arthritis (RA) by facilitating early diagnosis and ‘treat to the target.’ However, no gold standard biomarker has been identified for monitoring the disease activity in RA. Cytokines, a diverse group of small protein molecules secreted by peripheral blood mononuclear cells (PBMCs), play a pivotal role in pathogenesis and disease progression in RA. Research is currently underway to find out the applicability of cytokines as biomarkers in RA. This study aimed to quantify the PBMCs that secrete four types of cytokines; TNF-α, IL-1β, IL-10 and IL-17A in two cohorts of active RA patients (early RA patients and established RA patients), compared to healthy controls (HC), using the enzyme-linked immunosorbent spot (ELISPOT) assay, and to assess their association with measures of disease activity of RA. Patients were recruited from outpatient rheumatology clinics, and the disease activity was assessed using single and composite measures of disease activity. The cytokine expression was evaluated using freshly separated PBMCs from whole blood of RA patients using the ELISPOT assay. The number of PBMCs (counted as spot-forming cells (SFCs) per 105 PBMCs) that secreted the cytokine of interest were statistically significantly higher in early RA patients, compared to HC, for IL-17A (P
Databáze: OpenAIRE
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