Fibronectin expression is critical for liver fibrogenesis in vivo and in vitro
| Autor: | Fei Hou, Rui‑Xia Liu, En-tong Yi, Cheng‑Hong Yin, Yan Wen, Cheng Chi, Xiao Ya Liu, Li Jian Cui, Chunyun Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Liver Cirrhosis Male Cancer Research Biochemistry Cell Line 03 medical and health sciences Mice 0302 clinical medicine In vivo fibronectin Genetics Animals RNA Messenger Rats Wistar Molecular Biology Carbon Tetrachloride liver fibrosis biology Oncogene transforming growth factor-β Articles Molecular biology myofibroblast Fibronectins Fibronectin Blot 030104 developmental biology Real-time polymerase chain reaction Oncology Liver 030220 oncology & carcinogenesis biology.protein Cancer research Hepatic stellate cell Molecular Medicine Hepatic fibrosis Transforming growth factor |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | Increased fibronectin (FN) expression has an important role during liver fibrosis. The present study examined FN expression in rats subjected to carbon tetrachloride (CCl4)-induced liver fibrosis. In addition, the potential mechanisms underlying fibrogenesis were investigated by exposing hepatic stellate cells (HSCs) to transforming growth factor-β (TGF-β), which is a known inducer of myofibroblastic transformation of HSCs. Briefly, a rat model of liver fibrosis was created by administering intraperitoneal injections of CCl4. Furthermore, HSC-T6 cells were stimulated with increasing doses of recombinant TGF-β over 24 h. Hepatic fibrosis gradually increased following CCl4 administration in vivo. Western blotting and immunohistochemistry demonstrated that fibronectin (FN), TGF-β and α-smooth muscle actin (SMA) expression was increased following CCl4 injection, and the maximum expression levels were observed at 8 weeks. Once CCl4 treatment had been terminated, the expression levels of FN, TGF-β and α-SMA progressively declined to near baseline levels. Western blotting and quantitative polymerase chain reaction demonstrated that FN expression was gradually increased in response to TGF-β-stimulation of HSCs; maximum expression was achieved 12 h post-treatment (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|