Introgression of Thinopyrum elongatum DNA fragments carrying resistance to fusarium head blight into Triticum aestivum cultivar Chinese Spring is associated with alteration of gene expression
| Autor: | Farideh Tekieh, DaEun Lim, Kelsey Joustra, Aparna Haldar, Thérèse Ouellet, Fangpu Han, David Konkin, Margaret Balcerzak, George Fedak, Danielle Wolfe |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Fusarium
chromosome introgression expression génique marqueurs spécifiques de 7EL et de 7DL Triticum aestivum Introgression Locus (genetics) chemistry.chemical_compound Gene expression Genetics introgression chromosomique Cultivar 7EL- and 7DL-specific markers Molecular Biology biology Resistance (ecology) food and beverages Chromosome General Medicine Thinopyrum elongatum fusarium head blight resistance biology.organism_classification chemistry gene expression résistance à la fusariose de l’épi DNA Biotechnology |
| Zdroj: | Genome. 64:1009-1020 |
| ISSN: | 1480-3321 0831-2796 |
| DOI: | 10.1139/gen-2020-0152 |
| Popis: | The tall wheatgrass species Thinopyrum elongatum carries on the long arm of chromosome 7E, a locus that contributes strongly to resistance to fusarium head blight (FHB), a devastating fungal disease affecting wheat crops in all temperate areas of the world. Introgression of Th. elongatum 7E chromatin into chromosome 7D of wheat was induced by the ph1b mutant of CS. Recombinants between chromosome 7E and wheat chromosome 7D, induced by the ph1b mutation, were monitored by a combination of molecular markers and phenotyping for FHB resistance. Progeny of up to five subsequent generations derived from two lineages, 64-8 and 32-5, were phenotyped for FHB symptoms and genotyped using published and novel 7D- and 7E-specific markers. Fragments from the distal end of 7EL, still carrying FHB resistance and estimated to be less than 114 and 66 Mbp, were identified as introgressed into wheat chromosome arm 7DL of progeny derived from 64-8 and 32-5, respectively. Gene expression analysis revealed variation in the expression levels of genes from the distal ends of 7EL and 7DL in the introgressed progeny. The 7EL introgressed material will facilitate the use of the 7EL FHB resistance locus in wheat breeding programs. |
| Databáze: | OpenAIRE |
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