Steroidogenesis in amlodipine treated purified Leydig cells
| Autor: | Ghulam Mustafa Lodhi, Waqas Hameed, Rabia Latif, Muhammad Shahbaz Aslam |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty medicine.drug_class chemistry.chemical_element Calcium channel blocker Calcium Biology Pharmacology Toxicology Calcium in biology Rats Sprague-Dawley In vivo Internal medicine medicine Animals Testosterone Amlodipine Calcium metabolism Leydig cell Leydig Cells Luteinizing Hormone Calcium Channel Blockers Rats medicine.anatomical_structure Endocrinology chemistry Pregnenolone medicine.drug |
| Zdroj: | Toxicology and applied pharmacology. 258(1) |
| ISSN: | 1096-0333 |
| Popis: | Drugs have been shown to adversely affect male fertility and recently anti-hypertensive drugs were added to the list. The anti-fertility effects of amlodipine, a calcium channel blocker, are well-illustrated in in vivo experiments but lack an in vitro proof. The present study was designed to experimentally elucidate the effects of amlodipine on Leydig cell steroidogenesis and intracellular calcium in vitro. Leydig cells of Sprague-Dawley rats were isolated and purified by Percoll. Cells were incubated for 3h with/without amlodipine in the presence/absence of LH, dbcAMP, Pregnenolone and 25-Hydroxycholesterol. Cytosolic calcium was measured in purified Leydig cells by fluorometric technique. The results showed significantly reduced (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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