Experimental rat model of chronic cerebral hypoperfusion-reperfusion mimicking normal perfusion pressure breakthrough phenomenon
| Autor: | Álvaro Zamarrón, Jose Fortes, Juan Manuel Revuelta, Gregorio Rodríguez-Boto, Raquel Gutiérrez-González, Jesús Vaquero |
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| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_specialty Mean arterial pressure Intracranial Pressure Hemodynamics Anastomosis Brain Ischemia 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Animals Humans Cerebral perfusion pressure Rats Wistar Intracranial pressure business.industry Arteriovenous malformation medicine.disease Rats Perfusion Cerebrovascular Circulation Reperfusion Cardiology Intracranial Arteriovenous Malformations Surgery Neurology (clinical) business 030217 neurology & neurosurgery |
| Zdroj: | Neurocirugia (English Edition). 31(5) |
| ISSN: | 2529-8496 |
| Popis: | Background Normal perfusion pressure breakthrough (NPPB) phenomenon is a major life-threatening complication that restricts the treatment of complex intracranial arteriovenous malformations. The aim of the study it to develop a rat model mimicking NPPB phenomenon that enables the evaluation of any therapy to prevent such complication. Methods Twenty Wistar male rats were randomly assigned to either a study or a control group. Study animals underwent an end-to-side left external jugular vein-common carotid artery anastomosis and ligation of bilateral external carotid arteries. Control animals only underwent ligation of bilateral external carotid arteries. All animals were sacrificed sixty days after the procedure. Hemodynamic parameters [mean arterial pressure (MAP), intracranial pressure (ICP) and cerebral perfusion pressure (CPP)], blood–brain barrier (BBB) permeability (measured by fluorescein staining) and histological features were then compared between both groups. Results A significant decrease in MAP and CPP was confirmed in the study group. An increase in ICP was also observed. A significant decrease in MAP and CPP was also present in the study group when comparing preoperative values with those recorded on days 0 (postoperative), 7 and 60. Fluorescein staining findings were consistent with signs of BBB disruption in study animals. Histological analysis demonstrated an increased number of pyknotic neurons in the ipsilateral hemisphere of rat brains included in the study group. Conclusion These results confirm that this model mimics a vascular steal state with chronic cerebral hypoperfusion comparable to patients with AVMs behavior and disruption of the BBB after fistula closure comparable to NPPB phenomenon disorders. |
| Databáze: | OpenAIRE |
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