Activation of CCK-B receptors elevates cytosolic Ca2+ levels in a pituitary cell line
| Autor: | Mikio Arisawa, Hiroyuki Yoshizaki, Tsutomu Kudoh, Hiromi Nagase, Mari Takamiya, Toshikazu Kuwahara, Atsuko Nakano |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
medicine.medical_specialty
Fura-2 Physiology Biology In Vitro Techniques Phosphatidylinositols digestive system Biochemistry Sincalide Cell Line Cellular and Molecular Neuroscience chemistry.chemical_compound Endocrinology Cytosol Anterior pituitary Pituitary Gland Anterior Internal medicine medicine Extracellular Cyclic AMP Animals Phosphatidylinositol Receptor Cholecystokinin digestive oral and skin physiology Receptor Cholecystokinin B Rats medicine.anatomical_structure chemistry Second messenger system Calcium Receptors Cholecystokinin Signal transduction hormones hormone substitutes and hormone antagonists |
| Zdroj: | Peptides. 14(4) |
| ISSN: | 0196-9781 |
| Popis: | Cytosolic Ca2+ levels ([Ca2+]i) in GH3 cells, a rat anterior pituitary tumor cell line, were monitored with fura-2 by fluorescence measurements. Cholecystokinin octapeptide (CCK-8) produced a transient elevation of [Ca2+]i. The elevation of [Ca2+]i by CCK-8 was inhibited by L-365,260, but not by devazepide. It was still observed when extracellular Ca2+ was eliminated, indicating that CCK-8 mobilizes Ca2+ from intracellular storage sites after interaction with CCK-B receptors. Cholecystokinin octapeptide increased the turnover of phosphatidylinositol, but it did not affect cyclic AMP levels. A possible involvement of phosphatidylinositol breakdown and calcium mobilization in the transduction system of CCK-B receptors in GH3 cells is suggested. |
| Databáze: | OpenAIRE |
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