Validation of molecular diagnostics for the detection of pseudocowpox virus

Autor: Fonseca Júnior, Antônio Augusto, de Freitas Passos, Érica Eustáquia, de Oliveira, Jaqueline Silva, Starling Rocha, Rafaella, Laguardia-Nascimento, Mateus, de Souza Trindade, Giliane
Rok vydání: 2022
Předmět:
Zdroj: Braz J Microbiol
ISSN: 1678-4405
1517-8382
Popis: The pseudocowpox virus (PCPV) is recognized for causing exanthematic lesions in cattle and humans. The diagnosis is important because it is a zoonosis and its clinical signs can be confused with foot-and-mouth disease, a high-impact bovine disease in livestock. The objective of this work is to validate a SYBR Green qPCR and a conventional PCR for virus detection in bovine samples. Detection limit tests, repeatability, reproducibility, sensitivity, and specificity were compared. When two analysts were compared, results demonstrated that training and pipetting influence the repeatability. The qPCR was more sensitive than conventional PCR but showed nonspecific reactions distinguishable by the melting curve. Both showed high repeatability and reproducibility.
Databáze: OpenAIRE
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