Genomic responses to 5-HT1a or 5-HT2a2c receptor activation is differentially regulated in four regions of rat brain
| Autor: | Nanda Tilakaratne, Eitan Friedman |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Male
Agonist medicine.medical_specialty Ketanserin medicine.drug_class Gene Expression Hippocampus Biology Piperazines Rats Sprague-Dawley Cerebellum Internal medicine Receptor Serotonin 5-HT2C medicine Animals Receptor Serotonin 5-HT2A RNA Messenger Receptor Genes Immediate-Early 5-HT receptor Cerebral Cortex Pharmacology 8-Hydroxy-2-(di-n-propylamino)tetralin musculoskeletal neural and ocular physiology 5-HT2 receptor Amphetamines Brain Genes fos Blotting Northern Receptor antagonist Corpus Striatum Rats Serotonin Receptor Agonists Cell biology Endocrinology Gene Expression Regulation nervous system Receptors Serotonin 5-HT1A receptor Serotonin Antagonists DNA Probes Receptors Serotonin 5-HT1 medicine.drug |
| Zdroj: | European Journal of Pharmacology. 307:211-217 |
| ISSN: | 0014-2999 |
| DOI: | 10.1016/0014-2999(96)00233-6 |
| Popis: | The functional profiles of brain 5-HT1A and 5-HT 2A c receptors were assessed by quantitating changes in the immediate early genes — c-fos, ngflc and tisl, following receptor activation with either 8-OH-DPAT (8-hydroxy-2-(di-n-propylamino)tetralin) or DOI (1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane). Stimulation of either class of 5-HT receptor elicited an induction of all three immediate early genes to varying extents in cortex, hippocampus and cerebellum, but not in striatum. The responses to 8-OH-DPAT peaked earlier than those to DOI. WAY 100135 (N-tertiobutyl-3-[4-(2-methoxyphenyl)-piperazinyl]-2-phenylpropanamide), the putative 5-HT1A receptor antagonist blocked the 8-OH-DPAT effect but not the responses to DOI. WAY 100135 by itself also elicited a relatively smaller genomic response. Ketanserin completely abolished the DOI-induced genomic responses. The results support the earlier findings that 5-HT1A receptor sites are abundant in frontal cortex and hippocampus. In addition, the robust genomic responses to 8-OH-DPAT as well as Northern hybridization with a cDNA probe for 5-HT1A mRNA in the cerebellum clearly implicate the functional expression of 5-HT1A receptors in this brain region. The responses to the 5-HT2 receptor agonist, DOI support a greater abundance of these receptors in the cortex, and relatively lower levels in hippocampus and cerebellum. The results suggest a differential induction pattern among the three immediate-early genes depending on the brain region and the 5-HT receptor subtype involved. |
| Databáze: | OpenAIRE |
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