Characterization of huJAM: evidence for involvement in cell-cell contact and tight junction regulation
| Autor: | Arthur J Huang, Randy J. Mrsny, Toni Klassen, Austin L. Gurney, Alane M. Gray, Holly L. Aaron, Jeffery Hooley, Richard A. DeMarco, Tony W. Liang, Sherman Fong, Daniel Tumas |
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| Rok vydání: | 2000 |
| Předmět: |
Cell Membrane Permeability
Junctional Adhesion Molecules Physiology Fluorescent Antibody Technique Gene Expression Inflammation CHO Cells Biology Cell junction Tight Junctions Mice Cell–cell interaction Cricetinae Cell Adhesion medicine Animals Humans RNA Messenger In Situ Hybridization Mice Inbred BALB C Tight junction Epithelial Cells Cell Biology Precipitin Tests Epithelium Cell biology medicine.anatomical_structure Caco-2 Immunoglobulin superfamily Female Caco-2 Cells medicine.symptom Cell Adhesion Molecules Junctional Adhesion Molecule A |
| Zdroj: | American Journal of Physiology-Cell Physiology. 279:C1733-C1743 |
| ISSN: | 1522-1563 0363-6143 |
| DOI: | 10.1152/ajpcell.2000.279.6.c1733 |
| Popis: | Cell-cell interactions of the mucosal epithelia are important for the maintenance and establishment of epithelial barrier function. During events of inflammation, such cell-cell interactions are often disrupted, resulting in a leaky epithelial barrier, which in turn can lead to various inflammatory and infective dysfunctions. Human junctional adhesion molecule (huJAM), found on the mucosal epithelia and vascular endothelia of many major organ systems, is a membrane glycoprotein which resolves to a doublet band of approximately 40 and approximately 37 kDa under SDS-PAGE analysis, representing differentially glycosylated forms of the same protein. huJAM was localized to the lateral membrane of Caco-2 cells (a human colonic epithelial cell line) monolayers, in an area basolateral of the epithelial tight junctions (TJ). Through functional and biochemical assays, we show huJAM to be able to homotypically associate and to participate in TJ restitution after trypsin-EDTA disruption. Furthermore, we also observed a migration of huJAM expression toward areas of cell-cell contacts during events of cell adhesion and monolayer formation. These qualities makes huJAM a likely player in the regulation of cell-cell contacts and the subsequent formation of TJs. |
| Databáze: | OpenAIRE |
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