Genomic instability of gold nanoparticle treated human lung fibroblast cells
| Autor: | Resham L Gurung, Soo-Ling Lo, Jasmine J. Li, Choon Nam Ong, Manoor Prakash Hande, Cheng Teng Ng, Lin-Yue Lanry Yung, Deny Hartono, Boon-Huat Bay |
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| Rok vydání: | 2011 |
| Předmět: |
Proteomics
DNA repair Biophysics Down-Regulation Gene Expression Metal Nanoparticles Bioengineering PDIA3 Biology Genomic Instability Cell Line Biomaterials medicine Humans Electrophoresis Gel Two-Dimensional Protein disulfide-isomerase Fibroblast Lung In Situ Hybridization Fluorescence NDUFS1 Reverse Transcriptase Polymerase Chain Reaction Proteins Chromosome Breakage Fibroblasts Molecular biology Up-Regulation Blot medicine.anatomical_structure Mechanics of Materials Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Ceramics and Composites Comet Assay Gold Chromosome breakage |
| Zdroj: | Biomaterials. 32(23) |
| ISSN: | 1878-5905 |
| Popis: | Gold nanoparticles (AuNPs) are one of the most versatile and widely researched materials for novel biomedical applications. However, the current knowledge in their toxicological profile is still incomplete and many on-going investigations aim to understand the potential adverse effects in human body. Here, we employed two dimensional gel electrophoresis to perform a comparative proteomic analysis of AuNP treated MRC-5 lung fibroblast cells. In our findings, we identified 16 proteins that were differentially expressed in MRC-5 lung fibroblasts following exposure to AuNPs. Their expression levels were also verified by western blotting and real time RT-PCR analysis. Of interest was the difference in the oxidative stress related proteins (NADH ubiquinone oxidoreductase (NDUFS1), protein disulfide isomerase associate 3 (PDIA3), heterogeneous nuclear ribonucleus protein C1/C2 (hnRNP C1/C2) and thioredoxin-like protein 1 (TXNL1)) as well as proteins associated with cell cycle regulation, cytoskeleton and DNA repair (heterogeneous nuclear ribonucleus protein C1/C2 (hnRNP C1/C2) and Secernin-1 (SCN1)). This finding is consistent with the genotoxicity observed in the AuNP treated lung fibroblasts. These results suggest that AuNP treatment can induce oxidative stress-mediated genomic instability. |
| Databáze: | OpenAIRE |
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