Human Monoclonal Antibodies toPseudomonas aeruginosaProduced by EBV-Transformed Cells
| Autor: | Mayumi Moriyama, Yoshiko Matsumoto, Hidenori Suzuki, Yuichi Okubo, Manami Sasaki, Toyoharu Hozumi |
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| Rok vydání: | 1987 |
| Předmět: |
Herpesvirus 4
Human medicine.drug_class Immunology Biology medicine.disease_cause Monoclonal antibody Microbiology Virus Mice Virology medicine Animals Humans Pseudomonas Infections Lymphocytes Gene Cell Line Transformed Mice Inbred ICR Pseudomonas aeruginosa Antibodies Monoclonal Antibodies Bacterial Immunoglobulin Classes Lymphoblastoid cell biology.protein Antibody |
| Zdroj: | Microbiology and Immunology. 31:959-966 |
| ISSN: | 0385-5600 |
| DOI: | 10.1111/j.1348-0421.1987.tb01328.x |
| Popis: | Numerous lymphoblastoid cell lines (LCLs) which secreted antibodies against Pseudomonas aeruginosa (all Fisher's immunotypes and Homma's immunotype 1) were established by Epstein-Barr virus (EBV)-transformation of lymphocytes. Five LCLs were established as long-term culture lines and their properties were determined. These LCLs produced monoclonal antibodies to Fisher's immunotype 1 and 4 and Homma's immunotype 1, and their immunoglobulin classes were IgM, IgG, and IgA. We found that three monoclonal antibodies (G3-1, H7-2, and E10-1) among them successfully protected mice from the corresponding immunotype of P. aeruginosa infection. Their protective dose (PD50) values were 0.5, 2.6, and 3.1 micrograms immunoglobulin/mouse. These human monoclonal antibodies against P. aeruginosa prepared by EBV-transformation method will be a valuable aid for the treatment for severe P. aeruginosa infections. |
| Databáze: | OpenAIRE |
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