Variations of circulating miRNA in paediatric patients with Heart Failure supported with Ventricular Assist Device: a pilot study

Autor: Chiara Caselli, Silvia Del Ry, Giuseppina Basta, Milena Rizzo, Manuela Cabiati, Arianna Di Molfetta, Letizia Pitto, Romina D'Aurizio, Maria Giovanna Trivella, Serena Del Turco, Rosetta Ragusa, Antonio Amodeo
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Circulating mirnas
Oncology
Male
medicine.medical_treatment
lcsh:Medicine
Pilot Projects
VAD therapy
030204 cardiovascular system & hematology
0302 clinical medicine
lcsh:Science
Paediatric patients
Cardiac device therapy
pediatric patients
Multidisciplinary
Hep G2 Cells
Factor VII
Prognosis
Real-time polymerase chain reaction
Child
Preschool
miRNAs
Female
Prothrombin
biological phenomena
cell phenomena
and immunity
medicine.medical_specialty
Down-Regulation
Real-Time Polymerase Chain Reaction
Article
03 medical and health sciences
Text mining
Internal medicine
microRNA
parasitic diseases
medicine
Humans
Circulating MicroRNA
cardiovascular diseases
Heart Failure
business.industry
Gene Expression Profiling
lcsh:R
Computational Biology
Infant
Thrombosis
medicine.disease
circulating miRNA
Gene expression profiling
MicroRNAs
030104 developmental biology
Ventricular assist device
Heart failure
lcsh:Q
Heart-Assist Devices
business
Biomarkers
Zdroj: Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
Scientific Reports
Scientific reports (Nature Publishing Group) 10 (2020). doi:10.1038/s41598-020-62757-7
info:cnr-pdr/source/autori:R. Ragusa (1) (2); A. Di Molfetta (3); R. D'Aurizio (4); S. Del Turco (2); M. Cabiati (2); S. Del Ry (2); G. Basta (2); L. Pitto (2); A. Amodeo (3); M. G: Trivella (2); M. Rizzo (2); C. Caselli (2)/titolo:Variations of circulating miRNA in paediatric patients with Heart Failure supported with Ventricular Assist Device: a pilot study/doi:10.1038%2Fs41598-020-62757-7/rivista:Scientific reports (Nature Publishing Group)/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume:10
ISSN: 2045-2322
DOI: 10.1038/s41598-020-62757-7
Popis: Circulating miRNAs (c-miRNAs) are promising biomarkers for HF diagnosis and prognosis. There are no studies on HF pediatric patients undergoing VAD-implantation. Aims of this study were: to examine the c-miRNAs profile in HF children; to evaluate the effects of VAD on c-miRNAs levels; to in vitro validate putative c-miRNA targets. c-miRNA profile was determined in serum of HF children by NGS before and one month after VAD-implant. The c-miRNA differentially expressed were analyzed by real time-PCR, before and at 4 hrs,1,3,7,14,30 days after VAD-implant. A miRNA mimic transfection study in HepG2 cells was performed to validate putative miRNA targets selected through miRWalk database. Thirteen c-miRNAs were modified at 30 days after VAD-implant compared to pre-VAD at NSG, and, among them, six c-miRNAs were confirmed by Real-TimePCR. Putative targets of the validated c-miRNAs are involved in the hemostatic process. The in vitro study confirmed a down-regulatory effect of hsa-miR-409-3p towards coagulation factor 7 (F7) and F2. Of note, all patients had thrombotic events requiring pump change. In conclusion, in HF children, the level of six c-miRNAs involved in the regulation of hemostatic events changed after 30 days of VAD-treatment. In particular, the lowering of c-miR-409-3p regulating both F7 and F2 could reflect a pro-thrombotic state after VAD-implant.
Databáze: OpenAIRE
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