Loss of mismatch repair protein immunostaining in colorectal adenomas from patients with hereditary nonpolyposis colorectal cancer
| Autor: | Annika Lindblom, Kristina Lagerstedt, Mef Nilbert, Britta Halvarsson, Leif Johansson |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Adenoma
Adult Male congenital hereditary and neonatal diseases and abnormalities Pathology medicine.medical_specialty DNA Repair Base Pair Mismatch MLH1 Pathology and Forensic Medicine Proto-Oncogene Proteins PMS2 Humans Medicine neoplasms Adaptor Proteins Signal Transducing Aged Mismatch Repair Endonuclease PMS2 Adenosine Triphosphatases business.industry Nuclear Proteins nutritional and metabolic diseases Mismatch Repair Protein Middle Aged medicine.disease Colorectal Neoplasms Hereditary Nonpolyposis Immunohistochemistry digestive system diseases Neoplasm Proteins DNA-Binding Proteins MSH6 DNA Repair Enzymes MutS Homolog 2 Protein MSH2 Cancer research Female DNA mismatch repair Carrier Proteins Colorectal Neoplasms MutL Protein Homolog 1 business Immunostaining |
| Zdroj: | Modern Pathology. 18:1095-1101 |
| ISSN: | 0893-3952 |
| DOI: | 10.1038/modpathol.3800392 |
| Popis: | Colorectal adenomas occur at younger age, at increased frequency and have a greater tendency for malignant transformation in patients with hereditary nonpolyposis colorectal cancer (HNPCC). We performed immunostaining for the mismatch repair proteins MLH1, PMS2, MSH2 and MSH6 in 35 colorectal adenomas from 26 patients with HNPCC and identified loss of immunostaining in 23/35 (0.66) adenomas. Loss of mismatch repair protein immunostaining was particularly frequent in large (5 mm) (14/16) and proximally located (13/15) adenomas, whereas the gene mutated--MLH1 or MSH2--and the type of mutation did not seem to affect the results. We conclude that loss of mismatch repair protein immunostaining is detected at a lower rate in adenomas than in carcinomas associated with HNPCC. Adenomatous tissue can thus be used for immunostaining of mismatch repair proteins in clinical investigations of HNPCC, but whereas loss of immunostaining may pinpoint the gene affected and thereby guide mutation analysis, retained staining cannot exclude that the adenoma developed as part of the syndrome due to reduced sensitivity. However, the analysis has a greater chance of being informative if large and proximally located adenomas are selected. |
| Databáze: | OpenAIRE |
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