A pair of long intergenic non-coding RNA LINC00887 variants act antagonistically to control Carbonic Anhydrase IX transcription upon hypoxia in tongue squamous carcinoma progression
| Autor: | Xiangting Wang, Guang-Yan Yu, Wangxiao Xia, Li-Ling Wu, Guodong Shen, Qianxi Zhan, Tao Shen, Fanghong Shao, Wei Wang, Zixuan Yang, Qing-Peng Kong, Lehua Cheng, Xuehan Zhang, Yan Zhang, Huafeng Zhang, Sai-Nan Min, Zhiyu Wang |
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| Rok vydání: | 2021 |
| Předmět: |
QH301-705.5
Physiology Carbonic anhydrase 9 Plant Science Biology Hypoxia-induced factor General Biochemistry Genetics and Molecular Biology Tongue Structural Biology Transcription (biology) Cell Line Tumor Humans Biology (General) Carbonic Anhydrase IX Hypoxia Gene Ecology Evolution Behavior and Systematics Cancer Alternative promoter DNA methylation Cell Biology Carbonic Anhydrase 9 Non-coding RNA Long non-coding RNA Tongue Neoplasms Squamous carcinoma Tumor progression Carcinoma Squamous Cell Cancer research RNA Long Noncoding General Agricultural and Biological Sciences Genome-Wide Association Study Research Article Alternative splicing Long noncoding RNA Developmental Biology Biotechnology |
| Zdroj: | BMC Biology, Vol 19, Iss 1, Pp 1-22 (2021) BMC Biology |
| ISSN: | 1741-7007 |
| Popis: | Background Long noncoding RNAs (lncRNAs) are important regulators in tumor progression. However, their biological functions and underlying mechanisms in hypoxia adaptation remain largely unclear. Results Here, we established a correlation between a Chr3q29-derived lncRNA gene and tongue squamous carcinoma (TSCC) by genome-wide analyses. Using RACE, we determined that two novel variants of this lncRNA gene are generated in TSCC, namely LINC00887_TSCC_short (887S) and LINC00887_TSCC_long (887L). RNA-sequencing in 887S or 887L loss-of-function cells identified their common downstream target as Carbonic Anhydrase IX (CA9), a gene known to be upregulated by hypoxia during tumor progression. Mechanistically, our results showed that the hypoxia-augmented 887S and constitutively expressed 887L functioned in opposite directions on tumor progression through the common target CA9. Upon normoxia, 887S and 887L interacted. Upon hypoxia, the two variants were separated. Each RNA recognized and bound to their responsive DNA cis-acting elements on CA9 promoter: 887L activated CA9’s transcription through recruiting HIF1α, while 887S suppressed CA9 through DNMT1-mediated DNA methylation. Conclusions We provided hypoxia-permitted functions of two antagonistic lncRNA variants to fine control the hypoxia adaptation through CA9. |
| Databáze: | OpenAIRE |
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