A comparative genomics approach for identifying host-range determinants in Streptococcus thermophilus bacteriophages
| Autor: | João M. Monteiro, Mariana G. Pinho, Paula Szymczak, Finn K. Vogensen, Thomas Janzen, Sergio R. Filipe, Martin Holm Rau, Ahmad A. Zeidan |
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| Přispěvatelé: | DCV - Departamento de Ciências da Vida, Molecular, Structural and Cellular Microbiology (MOSTMICRO), UCIBIO - Applied Molecular Biosciences Unit, Instituto de Tecnologia Química e Biológica António Xavier (ITQB) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Streptococcus thermophilus Streptococcus Phages Operon viruses lcsh:Medicine Genomics Genome Viral Genome Article Host Specificity 03 medical and health sciences 0302 clinical medicine Phylogenetics Genotype Bacteriophages Super-resolution microscopy lcsh:Science General Phylogeny Comparative genomics Genetics Genetic diversity Multidisciplinary biology lcsh:R biology.organism_classification 030104 developmental biology lcsh:Q 030217 neurology & neurosurgery |
| Zdroj: | Szymczak, P, Rau, M H, Monteiro, J M, Pinho, M G, Filipe, S R, Vogensen, F K, Zeidan, A A & Janzen, T 2019, ' A comparative genomics approach for identifying host-range determinants in Streptococcus thermophilus bacteriophages ', Scientific Reports, vol. 9, 7991 . https://doi.org/10.1038/s41598-019-44481-z Scientific Reports Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Scientific Reports, Vol 9, Iss 1, Pp 1-14 (2019) |
| DOI: | 10.1038/s41598-019-44481-z |
| Popis: | The authors would like to thank: Mimi Birkelund, Ditte E. Christiansen, Goncalo Covas, and Denise Coutinho for their help in collecting data; Ana Rute Neves for valuable discussions; Witold Kot and Lars H. Hansen for sequencing of phage genomes at Aarhus University (Roskilde, Denmark). This work is part of an Industrial PhD Project funded by Innovation Fund Denmark and Chr. Hansen A/S, grant no. 4135-00104B. Comparative genomics has proven useful in exploring the biodiversity of phages and understanding phage-host interactions. This knowledge is particularly useful for phages infecting Streptococcus thermophilus, as they constitute a constant threat during dairy fermentations. Here, we explore the genetic diversity of S. thermophilus phages to identify genetic determinants with a signature for host specificity, which could be linked to the bacterial receptor genotype. A comparative genomic analysis was performed on 142 S. thermophilus phage genomes, 55 of which were sequenced in this study. Effectively, 94 phages were assigned to the group cos (DT1), 36 to the group pac (O1205), six to the group 5093, and six to the group 987. The core genome-based phylogeny of phages from the two dominating groups and their receptor binding protein (RBP) phylogeny corresponded to the phage host-range. A role of RBP in host recognition was confirmed by constructing a fluorescent derivative of the RBP of phage CHPC951, followed by studying the binding of the protein to the host strain. Furthermore, the RBP phylogeny of the cos group was found to correlate with the host genotype of the exocellular polysaccharide-encoding operon. These findings provide novel insights towards developing strategies to combat phage infections in dairies. publishersversion published |
| Databáze: | OpenAIRE |
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