Slow freezing versus vitrification for the cryopreservation of zebrafish (Danio rerio) ovarian tissue
Autor: | Tiantian Zhang, Francielli Weber Santos, Helen Tais da Rosa, José Cláudio Fonseca Moreira, Juliana Bernera Ramalho, Lis Santos Marques, Aryele Pinto Izaguirry, Rômulo Batista Rodrigues, Ana Amélia Nunes Fossati, Danilo Pedro Streit |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
food.ingredient Cryoprotectant lcsh:Medicine Mitochondrion Antioxidants Article Cryopreservation Andrology 03 medical and health sciences chemistry.chemical_compound Cryoprotective Agents 0302 clinical medicine food Yolk Freezing Animals Vitrification lcsh:Science Zebrafish Biophysical methods Animal biotechnology 030219 obstetrics & reproductive medicine Multidisciplinary Assay systems Chemistry Cell Membrane Ovary lcsh:R Mitochondria Staining 030104 developmental biology Oocytes Female Trypan blue lcsh:Q Reactive Oxygen Species Systems biology Intracellular DNA Damage |
Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019) Scientific Reports |
ISSN: | 2045-2322 |
DOI: | 10.1038/s41598-019-51696-7 |
Popis: | The aim of the present study was to compare the efficiency of vitrification and slow freezing techniques for the cryopreservation of zebrafish ovarian tissue containing immature follicles. In Experiment 1, assessment of cell membrane integrity by trypan blue exclusion staining was used to select the best cryoprotectant solution for each cryopreservation method. Primary growth (PG) oocytes showed the best percentage of membrane integrity (63.5 ± 2.99%) when SF4 solution (2 M methanol + 0.1 M trehalose + 10% egg yolk solution) was employed. The vitrification solution, which presented the highest membrane integrity (V2; 1.5 M methanol + 5.5 M Me2SO + 0.5 M sucrose + 10% egg yolk solution) was selected for Experiment 2. Experiment 2 aimed to compare the vitrification and slow freezing techniques in the following parameters: morphology, oxidative stress, mitochondrial activity, and DNA damage. Frozen ovarian tissue showed higher ROS levels and lower mitochondrial activity than vitrified ovarian tissue. Ultrastructural observations of frozen PG oocytes showed rupture of the plasma membrane, loss of intracellular contents and a large number of damaged mitochondria, while vitrified PG oocytes had intact mitochondria and cell plasma membranes. We conclude that vitrification may be more effective than slow freezing for the cryopreservation of zebrafish ovarian tissue. |
Databáze: | OpenAIRE |
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