Quercetin Protects Against Lipopolysaccharide-Induced Intestinal Oxidative Stress in Broiler Chickens through Activation of Nrf2 Pathway

Autor: Lianyu Yang, Gaoqing Xu, Wenfa Lu, Lei Sun, Meng Li, Yangyunyi Dong
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Lipopolysaccharides
Male
Pharmaceutical Science
Mitochondrion
medicine.disease_cause
p38 Mitogen-Activated Protein Kinases
Analytical Chemistry
quercetin
chemistry.chemical_compound
0302 clinical medicine
Malondialdehyde
Drug Discovery
oxidative stress
Phosphorylation
Extracellular Signal-Regulated MAP Kinases
broiler chickens
chemistry.chemical_classification
biology
Glutathione peroxidase
digestive
oral
and skin physiology
Mitochondria
Intestines
Chemistry (miscellaneous)
030220 oncology & carcinogenesis
Molecular Medicine
gut
lipids (amino acids
peptides
and proteins)
Quercetin
Signal Transduction
animal structures
MAP Kinase Signaling System
NF-E2-Related Factor 2
SOD2
Protective Agents
digestive system
Article
Nrf2
Superoxide dismutase
lcsh:QD241-441
03 medical and health sciences
lcsh:Organic chemistry
medicine
Animals
Physical and Theoretical Chemistry
Reactive oxygen species
Organic Chemistry
JNK Mitogen-Activated Protein Kinases
Molecular biology
MAPK
030104 developmental biology
chemistry
Gene Expression Regulation
biology.protein
Reactive Oxygen Species
Chickens
Oxidative stress
Zdroj: Molecules
Volume 25
Issue 5
Molecules, Vol 25, Iss 5, p 1053 (2020)
ISSN: 1420-3049
DOI: 10.3390/molecules25051053
Popis: We investigated the potential ability of quercetin to protect against lipopolysaccharide (LPS)-induced intestinal oxidative stress in broiler chickens and the potential role of the Nrf2 (nuclear factor erythroid 2-related factor 2) signaling pathway. One-day-old broiler chickens (n = 240) were randomized into four groups: saline-challenged broiler chickens fed a basal diet (Con), LPS-challenged broiler chickens on a basal diet (LPS), and LPS-treated broiler chickens on a basal diet containing either 200 or 500 mg/kg of quercetin (Que200+LPS or Que500+LPS). Quercetin (200 mg/kg) significantly alleviated LPS-induced decreased duodenal, jejunal, and illeal villus height and increased the crypt depth in these regions. Quercetin significantly inhibited LPS-induced jejunal oxidative stress, including downregulated reactive oxygen species (ROS), malondialdehyde (MDA), and 8-hydroxy-2&prime
deoxyguanosine (8-OHdG) levels, and it upregulated superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels. Quercetin relieved LPS-induced jejunal mitochondria damage and upregulated mitochondrial DNA copy number-related gene expression, including cytochrome c oxidase subunit 1 (COX1), ATP synthase F0 subunit 6 (ATP6), and NADH dehydrogenase subunit 1 (ND1). Quercetin attenuated the LPS-induced inhibition of Nrf2 activation, translocation, and downstream gene expression, including heme oxygenase-1 (HO-1), NAD (P) H dehydrogenase quinone 1 (NQO1), and manganese superoxide dismutase (SOD2). Additionally, quercetin attenuated the LPS-inhibition of c-Jun N-terminal kinase (JNK), Extracellular Regulated protein Kinases (ERK), and p38MAPK (p38) phosphorylation in the MAPK pathway. Thus, quercetin attenuated LPS-induced oxidative stress in the intestines of broiler chickens via the MAPK/Nrf2 signaling pathway.
Databáze: OpenAIRE
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