Plasmodium falciparum Drug-Resistant Haplotypes and Population Structure in Postearthquake Haiti, 2010
| Autor: | David A. Townes, Dragan Ljolje, Curtis S. Huber, Michelle A. Chang, Sheila Okoth, Sean M. Griffing, Naomi W. Lucchi, Meredith McMorrow, Roland Oscar, Lindsay Morton, Venkatachalam Udhayakumar, Jacques Boncy, John W. Barnwell |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Plasmodium falciparum 030106 microbiology 030231 tropical medicine Drug Resistance Protozoan Proteins Drug resistance law.invention Antimalarials 03 medical and health sciences 0302 clinical medicine Chloroquine law Virology Sulfadoxine parasitic diseases Earthquakes Prevalence medicine Humans Malaria Falciparum Genotyping Alleles Polymerase chain reaction Genetics biology Haplotype Membrane Transport Proteins Articles biology.organism_classification medicine.disease Haiti Drug Combinations Genetics Population Pyrimethamine Infectious Diseases Haplotypes Mutation Microsatellite Parasitology Multidrug Resistance-Associated Proteins Malaria Microsatellite Repeats medicine.drug |
| Zdroj: | The American Journal of Tropical Medicine and Hygiene. 95:811-816 |
| ISSN: | 1476-1645 0002-9637 |
| Popis: | Chloroquine (CQ) remains the first-line treatment of malaria in Haiti. Given the challenges of conducting in vivo drug efficacy trials in low-endemic settings like Haiti, molecular surveillance for drug resistance markers is a reasonable approach for detecting resistant parasites. In this study, 349 blood spots were collected from suspected malaria cases in areas in and around Port-au-Prince from March to July 2010. Among them, 121 samples that were Plasmodium falciparum positive by polymerase chain reaction were genotyped for drug-resistant pfcrt, pfdhfr, pfdhps, and pfmdr1 alleles. Among the 108 samples that were successfully sequenced for CQ resistant markers in pfcrt, 107 were wild type (CVMNK), whereas one sample carried a CQ-resistant allele (CVIET). Neutral microsatellite genotyping revealed that the CQ-resistant isolate was distinct from all other samples in this study. Furthermore, the remaining parasite specimens appeared to be genetically distinct from other reported Central and South American populations. |
| Databáze: | OpenAIRE |
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